Specific Measurement of Putrescine with Putrescine Oxidase and Aminobutyraldehyde Dehydrogenase(Biological Chemistry)

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概要

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• A simple and rapid spectrophotometric method, based upon the UV absorption of NADH, for the measurement of putrescine by the combined use of aminobutyraldehyde dehydrogenase (EC 1.2.1.19) and putrescine oxdase (EC 1.4.3.10) was established. Simplified purification procedures for the analytical use of aminobutyraldehyde dehydrogenase of Pseudomonas putida and putrescine oxidase of a newly isolated Gram-positive soil bacterium were developed. When aminobutyraldehyde dehydrogenase alone was used, the amount of 4-aminobutyraldehyde and the absorbance of NADH formed during the enzyme reaction has a precisely linear relationship in the range from 2 to 80 nmol of 4-aminobutyraldehyde. A comparable accuracy and sensitivity was obtained when a combination of putrescine oxidase and aminobutyraldehyde dehydrogenase was used to measure putrescine.

• 社団法人日本農芸化学会の論文
• 1988-11-23

著者

• Yorifuji Takamitsu

  Department Of Baioscience And Biotechnology Shinshu University

• Yorifuji Takamitsu

  Department Of Agricultural And Biological Chemistry Shinshu University

• Shimizu E

  Department Of Bioscience And Biotechnology Shinshu University

• Shimizu Eiichi

  Department Of Anatomy Wakayama Medical College

• Yorifuji T

  Department Of Bioscience And Biotechnology Shinshu University

• Yorifuji T

  Department Of Baioscience And Biotechnology Shinshu University

• TABATA Yasushi

  Department of Bioscience and Biotechnology, Shinshu University

• HAYAKAWA Ryuji

  Department of Biochemistry and Cell Biology, Shinshu University

• Tabata Yasushi

  Department Of Bioscience And Biotechnology Shinshu University

• Hayakawa Ryuji

  Department Of Biochemistry And Cell Biology Shinshu University

• YORIFUJI Takamitsu

  Department of Agricaltural Chemistry, Faculty of Agriculture, Shinshu University

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