Diguanidinobutane (Arcaine) Degradation in Rhodococcus sp. C-x

概要

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A nocardioform bacterium Rhodococcus sp. C-x, isolated from soil grew well on 1, 4-diguanidinobutane (DGB ; arcaine) as the sole nitrogen source. DGB was degraded to putrescine by a pathway with carbamoylagmatine (1-guanidino-4-ureidobutane), agmatine (1-amino-4-guanidinobutane), and carbamoylputrescine (1-amino-4-ureidobutane) as the intermediates. The enzymes of this pathway were partially purified from cells grown on DGB or agmatine and examined. The first and third steps of the degradation pathway of DGB were catalyzed by agmatine deiminase (EC 3. 5. 3. 12), which had relative activities of 100% and 30% toward agmatine and DGB, respectively. The second and fourth steps were catalyzed by carbamoylputrescine amidase (EC 3. 5. 1. 53), which had relative activities of 100%, 63%, and 12% toward carbamoylputrescine, carbamoylagmatine, and N-carbamoyl-β-alanine, respectively.
社団法人日本農芸化学会の論文
1994-05-23