Plant Regeneration of Peppermint, Mentha piperita, from the Hairy Roots Generated from Microsegment Infected with Agrobacterium rhizogenes
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概要
- 論文の詳細を見る
Microsegments from peppermint, Mentha piperita, were placed on supplemented Murashige & Skoog medium and infected with Agrobacterium rhizogenes. The hairy roots resulted from peppermint were cultured on various media in order to regenerate plants. The hairy roots formed a callus on Gamborg B5 medium containing 1μM 1-naphthaleneacetic acid (NAA), 10μM N-(2-chloro-4-pyridyl)-N’-phenylurea (4-CPPU), and 10% coconut powder in the dark. Shoots were regenerated from the calli cultured on 1/2 Murashige and Skoog medium containing 1μM NAA and 10μM 4-CPPU during a 16-h photoperiod. All plants were recovered following rooting of the shoots in B5 medium without hormones. The polymerase chain reaction (PCR) analysis of the genomic DNA showed that all regenerated plants had the region from the rolA to rolB gene.
- 日本植物細胞分子生物学会の論文
- 2003-06-01
著者
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Minami M
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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Minami Mineo
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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Inoue Fumihide
The United Graduate School Of Agricultural Science Gifu University (shinshu University)
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Tabuchi Akira
Department Of Biology Pennsylvania State University
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Tabuchi Akira
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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SUGIURA Hiroyuki
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University
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KARASAWA Den-ei
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University
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Tabuchi A
Department Of Biology Pennsylvania State University
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Sugiura Hiroyuki
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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Karasawa Den-ei
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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TABUCHI Akira
Department of Agricultural and Biological Chemistry, Shinshu University
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