Digital Microscopy : A Useful Technique for Measuring Root Elongation in Solution
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概要
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Decreased root elongation and rupture of outer cells, major effects of soluble aluminum (Al), may be studied using digital microscopy with little interference by the experimental technique. Single roots of 3-d-old mungbean (Vigna radiata L.) or soybean (Glycine max (L.) Merr.) seedlings were marked with activated charcoal particles and grown for ca. 2h in 500mL 1mM CaCl_2 solution at pH 6, followed by the imposition of an Al treatment. A digital image at 25-time magnification was recorded every 5 min for up to 7h. Examination of the digital images showed that Al exerted its rhizotoxic effects rapidly (ca. 20-50 min) by reducing cell expansion in the elongation zone. Rupture of epidermal and outer cortical cells occurred later (after〓4h) and closer to the root tip. Digital microscopy has a number of inherent benefits and problems, but is overall a valuable technique that may find wide use in studies on root growth.
- 社団法人日本土壌肥料学会の論文
著者
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Nishizawa Naoko
Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Nishizawa Naoko
Department Of Global Agricultural Science The University Of Tokyo
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Nishizawa Naoko
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Blamey Frederick
Department of Global Agricultural Science, The University of Tokyo
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Yoshimura Etsuro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Blamey Frederick
Department Of Global Agricultural Science The University Of Tokyo:(present Address) School Of Land A
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Yoshimura Etsuro
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Yoshimura Etsuro
Department of Agricultural Chemistry, The University of Tokyo
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