Mutational Analysis of the Length of the J3/4 Domain of Escherichia coli Ribonuclease P Ribozyme
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概要
- 論文の詳細を見る
We prepared a series of length variants of the J3/4 domain of Escherichia coli ribonuclease P (RNase P) ribozyme: the four-base long J3/4 domain (A62G63G64A65) was replaced with GGA (denoted ΔA), GA (ΔAG), A (ΔAGG), AAGGA (ΣA), AAAGGA (ΣAA), and AAAAGGA (ΣAAA). The results indicated that truncating and inserting operations of the J3/4 domain drastically reduced ribozyme activity (WT>>ΣAA>ΣA>ΣAAA>>ΔAG>ΔA, ΔAGG), but did not affect the cleavage site selection of a substrate by the ribozyme. The reduced ribozyme activity of each mutant was rescued to some extent by the addition of a high concentration of magnesium ions. Our data indicate that the conserved AGGA sequence was important for efficient ribozyme reactions, and suggested that the length mutations affected ribozyme activity through metal ion binding steps.
- 社団法人 日本農芸化学会の論文
- 2004-12-23
著者
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Tanaka T
Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center
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Tanaka Takaaki
Graduate School Of Science And Technology Niigata University
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Kikuchi Y
Div. Of Life Sci. And Biotechnology Dep. Of Ecological Engineering Toyohashi Univ. Of Technol.
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Kikuchi Yo
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Kikuchi Yo
Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O
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TANAKA Terumichi
Division of Life Science and Biotechnology, Department of Ecological Engineering, Toyohashi Universi
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HAGA Shinnosuke
Division of Bioscience and Biotechnology, Department of Ecological Engineering, Toyohashi University
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Haga Shinnosuke
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Tanaka T
Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University
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Kikuchi Y
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Tanaka Terumichi
Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O
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Tanaka Takaaki
Department Of Materials Science And Technology Faculty Of Engineering Niigata University
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Tanaka Terumichi
Graduate School of Science and Technology, Niigata University
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