Kinetics of Hyperprocessing Reaction of Human Tyrosine tRNA by Ribonuclease P Ribozyme from Escherichia coli(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
Human tyrosine tRNA and fly alanine, histidine, and initiator methionine tRNAs are generally cleavable internally by bacterial ribonuclease P ribozyme. The unusual internal cleavage reaction of tRNA, called hyperprocessing, occurs when the cloverleaf structure of the tRNA molecule is denatured to form a double-hairpin-like structure. The hyperprocessing reaction of these tRNAs requires magnesium ions. We analyzed details of this reaction using human tyrosine tRNA and Escherichia coli RNase P ribozyme. The usual processing reaction occurred efficiently with magnesium at 5 mM, but for the hyperprpocessing reaction, higher concentrations were needed. With such high concentrations, hyperprocessing cleaved both mature tRNA and tRNA precursor as substrates. When mature tRNA was the substrate, the apparent K_M was almost the same as in the usual reaction, but k_<cat> was smaller. These results indicated that the occurrence of hyperprocessing depends on the magnesium ion concentration, and suggested that magnesium ions contribute to the recognition of the shape of the substrate by bacterial RNase P enzymes.
- 社団法人日本農芸化学会の論文
- 2002-09-23
著者
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Ando T
Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And
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Ando Tomoaki
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Hori Yoshiaki
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Hori Yoshiaki
Laboratory Of Postharvest Science Devision Of Bioproduction System Science Departmetn Of Bioproducti
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Hori Y
Laboratory Of Postharvest Science Division Of Bioproduction System Science Department Of Bioproducti
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Ando T
Graduate School Of Bio-applications And Systems Engineering (base) Tokyo University Of Agriculture A
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Kikuchi Y
Div. Of Life Sci. And Biotechnology Dep. Of Ecological Engineering Toyohashi Univ. Of Technol.
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Kikuchi Yo
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Kikuchi Yo
Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O
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TANAKA Terumichi
Division of Life Science and Biotechnology, Department of Ecological Engineering, Toyohashi Universi
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Hori Y
Kyushu Institute Of Design
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Tanaka T
Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University
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Kikuchi Y
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Tanaka Terumichi
Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O
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Ando Tetsu
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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