Monoclonal Antibody against Bovine Lactoferricin® and Its Epitopic Site
スポンサーリンク
概要
- 論文の詳細を見る
Bovine lactoferricin® (LFcin B) is a strong antimicrobial peptide derived from N-lobe of lactoferrin. To study the immunochemical and structural properties of LFcin B, monoclonal antibody (mAb) was prepared and the amino acid sequence concerning with the binding to mAb has becn identified. Mice injected with LFcin B showed no production of antibody specific to this peptide, whereas those with LFcin B-KLH conjugate produced anti-LFcin B antibodies. None of the mAb reacted with bovine lactoferrin C-lobe, human lactoferrin or LFcin H. By the reactivity of the mAb against the peptides synthesized on cellulose membranes using SPOTs™ and against chemically modified derivatives of LFcin B, the antigenic determinant of LFcin B was identified to be the sequence of "QWR".牛ラクトフェリンのN-末端に近い25残基からなるペプチド，ラクトフェリシン (LFcin) は非常に強い抗菌活性を示す．LFcinの多様な機能の発現機作を解明する一手段として，抗LFcinモノクローナル抗体 (mAb) を作成した．LFcinはKLHにカップリングさせてマウスを免疫した．得られたmAbは化学合成したLFcinとも反応性を示した．膜上でのべプチド合成システムSPOTsによる判定結果，およびアミノ酸側鎖の化学修飾による結果と併せ，抗LFcin mAbの結合する部位はQWRと推定された.
- The Japanese Society of Veterinary Science = 日本獣医学会の論文
- 1996-12-25
著者
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Nam Myoung
Korea Research Institute of Bioscience and Biotechnology
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NAM Myoung
北大・農
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Kumura H
Hokkaido Univ. Hokkaido Jpn
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Kumura Haruto
北海道大学 農学研究科畜産科学科
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Kumura Haruto
Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr
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Kumura Haruto
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
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Nam Myoung
Dairy Science Laboratory College Of Agriculture Chungnam National University
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Shimazaki K
Hokkaido Univ. Hokkaido Jpn
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Shimazaki Kei-ichi
Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr
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MIKAWA Katsuhiko
Laboratory of Dairy Science, Division of Bioresources and Product Science
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OMATA Yoshitaka
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine
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原川 信二
帯広畜産大学原虫病研究センター:(株)白寿生科学研究所
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原川 信二
帯広畜産大学原虫病研究センター:(株)白寿生科学研究所研究開発部
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Igarashi Ikuo
The Research Center For Protozoan Molecular Immunology Obihiro University Of Agriculture And Veterin
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Omata Yoshitaka
Department Of Veterinary Physiology Obihiro University Of Agriculture And Veterinary Medicine
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Harakawa S
Department Of Veterinary Physiology Obihiro University Of Agriculture And Veterinary Medicine
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Harakawa Shinji
Research Unit For Functional Genomics National Research Center For Protozoan Diseases Obihiro Univer
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Tanaka T
Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University
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Mikawa Katsuhiko
Laboratory Of Dairy Science Division Of Bioresources And Product Science
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Saito A
Tokyo Dental Coll Tokyo Jpn
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Suzuki N
The Research Center For Protozoan Molecular Immunology Obihiro University Of Agriculture And Veterin
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Shimazaki K
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
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