Susceptibilities against Bovine Lactoferrin with Microorganisms Isolated from Mastitic Milk

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概要

• 論文の詳細を見る

• Antibacterial effects of bovine lactoferrin were studied in vitro against microorganisms isolated from mastitic milk in Tokachi area, Hokkaido, Japan. Microorganisms isolated were Eschelichia coli (11 isolates), Klebsiella pneumoniae (5 isolates), enterococci (8 isolates), Staphylococcus aureus (10 isolates), coagulase negative staphylococci (CNS, 13 isolates), streptococci (11 isolates), Prototheca zopfii (7 isolates) and yeast-like fungi (9 isolates). Lactoferrin has been known as a multifunctional protein and its antimicrobial effect is one of the most essential function of it. In order to compare their susceptibilities against lactoferrin, the minimal inhibitory concentration values were estimated by a microplate assay method using 96-well microplate, which involved measuring the optical density of the cultures. Prototheca zopfii was highly sensitive to bovine lactoferrin and complete inhibition of this microorganism was observed even at the low concentration of 7 μg/ml. On the other hand, E. coli and enterococci showed resistance against lactoferrin action and staphylococci showed strain-dependent resistance.臨床型乳房炎乳からE. coli（11株），K. pneumoniae（5株），enterococci（8株）， S. aureus（10株），CNS（13株），streptococci（11株），P. zopfii（7株），yeast-like fungi（9株）を分離し，ラクトフェリンに対する感受性を比較した．その結果，P. zopfiiがラクトフェリンによって最も増殖抑制を受け易かった．一方，E. coliやenterococciはラクトフェリンの影響を受けにくく，またS. aureusは菌株によって感受性の有無が分かれた．

• The Japanese Society of Veterinary Science = 日本獣医学会の論文

著者

• Kumura H

  Hokkaido Univ. Hokkaido Jpn

• Kumura Haruto

  Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr

• Kumura Haruto

  Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University

• Shimazaki K

  Hokkaido Univ. Hokkaido Jpn

• Shimazaki Kei-ichi

  Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University

• Shimazaki Kei-ichi

  Laboratory Of Dairy Science Research Group Of Animal Product Science Division Of Bioresources And Pr

• Lee Nai-Yuan

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokk

• Kawai Kazuhiro

  Seibu Veterinary Clinical Center

• Nakamura Ichiro

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokk

• Tanaka Tetsuya

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokk

• Shimazaki Kei-ichi

  Dairy Chemistry Research Laboratory Department Of Bioindustrial Chemistry Division Of Bioresource Ch

• Tanaka T

  Division Of Life Science And Biotechnology Department Of Ecological Engineering Toyohashi University

• Shimazaki K

  Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University

• LEE Nai-Yuan

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University

• SHIMAZAKI Kei-ichi

  Dairy Chemistry Laboratory, Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine

• KUMURA Haruto

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University

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