Substrate Shape Preference of Escherichia coli Ribonuclease P Ribozyme and Holo Enzyme Using Bottom-Half Part-Shifting Variants of Pre-tRNA
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概要
- 論文の詳細を見る
We showed previously that the bacterial ribonuclease P (RNase P) ribozyme has substrate shape preference depending on the concentrations of catalytically important magnesium ions. The ribozyme discriminates a canonical cloverleaf precursor tRNA from a hairpin RNA with a CCA-tag sequence at low concentrations of magnesium ions. By detailed analysis of the shape preference using the bottom-half part-shifting variants of a tRNA precursor, we showed that the RNAs in a T-shape structure can be substrates for the ribozyme reactions even at low concentrations of magnesium ions, and that the RNA in a natural L-shape is the best substrate for both the ribozyme and the holo enzyme. The results also showed that the position of the bottom-half part did not affect the cleavage site selection of a substrate by the enzyme. Our results are the first kinetic evidence to show the importance of the bottom-half part of tRNA molecule, and our result also showed that the holo enzyme can discriminate substrate shape as well as the ribozyme at low concentrations of metal ions.
- 社団法人 日本農芸化学会の論文
- 2005-10-23
著者
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Tanaka T
Bio-organic Chemistry Laboratory Institute For Fundamental Research Suntory Research Center
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Tanaka Takaaki
Graduate School Of Science And Technology Niigata University
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Kikuchi Y
Div. Of Life Sci. And Biotechnology Dep. Of Ecological Engineering Toyohashi Univ. Of Technol.
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Kikuchi Yo
Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O
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Nagai Yasuhiro
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Kikuchi Y
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Tanaka Terumichi
Division Of Bioscience And Biotechnology Department Of Ecological Engeneering Toyohashi University O
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Kikuchi Yo
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Tanaka Takaaki
Department Of Materials Science And Technology Faculty Of Engineering Niigata University
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Tanaka Terumichi
Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O
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Tanaka Terumichi
Graduate School of Science and Technology, Niigata University
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