Sex Pheromone Biosynthesis from ^<14>C-Hexadecanoic Acid in the Silkworm Moth(Biological Chemistry)

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概要

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• [16-^<14>C]Hexadecanoic acid in dimethyl sulfoxide was applied topically to a sex pheromone gland of the silkworm moth, Bombyx mori L. By the combination of normal-phase TLC and reversed-phase TLC or HPLC, bombykol (the sex pheromone of this species, (10E,12Z)-10,12-hexadecadien-1-ol) was purified, and its ^<14>C-incorporation was successfully counted without any contamination by other alcohol components in the gland. The highest incorporation ratio (ca. 1.5%) of the ^<14>C-acidinto bombykol was observed when a 1-day-old virgin female was treated 4 hr after lighting under a 16:8 light-dark cycle, the gland being excised for extraction 1 or 3 hr after the treatment. This reliable incorporation method is suggested to be useful for further investigations of the biosynthetic pathway. The ^<14>C-acid was also incorporated into other components in the pheromone gland, which have been proposed as precursors of bombykol biosynthesis, namely the (10E,12Z)-10,12-hexadecadienoic and (Z)-11-hexadecenoic acid moieties, hexadecan-1-ol, and (Z)-11-hexadecen-1-ol. The total recovery of radioactivity attained ca. 20%, including the unchanged ^<14>C-acid.

• 社団法人日本農芸化学会の論文
• 1988-01-23

著者

• Ando T

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Ando Tomoaki

  Division Of Bioscience And Biotechnology Department Of Ecological Engineering Toyohashi University O

• ARIMA Rika

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• UCHIYAMA Masaaki

  Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture an

• ANDO Tetsu

  Department of Plant Protection, Faculty of Agriculture, Tokyo University of Agriculture and Technolo

• HASE Takema

  Department of Plant Protection, Faculty of Agriculture, Tokyo University of Agriculture and Technolo

• FUNAYOSHI Atsunori

  Department of Plant Protection, Faculty of Agriculture, Tokyo University of Agriculture and Technolo

• Ando T

  Graduate School Of Bio-applications And Systems Engineering (base) Tokyo University Of Agriculture A

• Arima Rika

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Ando Tetsu

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Hase Takema

  Department Of Plant Protection Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• Uchiyama M

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Uchiyama M

  Department Of Plant Protection Faculty Of Agriculture Tokyo University Of Agriculture And Technology

• Uchiyama Masaaki

  Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo

• Funayoshi Atsunori

  Department Of Plant Protection Faculty Of Agriculture Tokyo University Of Agriculture And Technology

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