A Rice Lamina Inclination Test-A Micro-quantitative Bioassay for Brassinosteroids
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概要
- 論文の詳細を見る
A rice lamina inclination test that is simple and specific for brassinosteroids was used as a micro-quantitative bioassay for brassinolide 1 and its 6-keto congener, castasterone 2, in the concentration range of 5×10-5μg/ml to 5×10-3μg/ml, when uniform seedlings of the rice cultivars Arborio J-l and Nihonbare were selected. A phytohormone, indole-3-acetic acid (IAA), showed similar activity in this bioassay. Its lowest effective concentration, however, was 50μg/ml, about five orders of magnitude greater than that of brassinolide. Other phytohormones, abscisic acid (ABA) and the cytokinins kinetin and N6-benzyladenine, inhibited the lamina inclination of rice seedlings. The addition of a cytokinin reduced the promoting effect of brassinolide. Thus, the rice lamina inclination test can be used both as a micro-quantitative bioassay for brassinosteroids and as a method for detecting antibrassinolide compounds.
- 社団法人 日本農芸化学会の論文
著者
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Uchiyama Masaaki
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo
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Wada Kojiro
Department Of Agricultural Chemistry Nagoya University
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Nakamura Kenji
Department Of Anatomy Graduate School Of Medicine Osaka City University
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Morishita Tadashi
Department Of Orthodontics Faculty Of Dentistry Kyushu University
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Abe Hiroshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Mori Kenji
Department Of Agricultural Chemistry College Of Science And Technology The University Of Tokyo
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MARUMO Shingo
Department of Agricultural Chemistry
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