Inhibition of Lymphocyte Kinase Lck and Phosphatidylinositol 3-kinase by a Novel Immunosuppressant, Lymphostin(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Lck is a Src-family tyrosine kinase that is expressed predominantly in T cells, where it plays important roles in T-cell activation. Lymphostin was isolated from Streptomyces sp. as an inhibitor of Lck. As previously reported, lymphostin inhibited Lck (IC_<50> 0.05 μM) and the mixed lymphocyte reaction (IC_<50> 0.009 μM). We have now examined the mechanism of inhibition by lymphostin. Lymphostin inhibited protein-tyrosine kinase activity in Jurkat T cells, demonstrating the effectiveness of the compound at the cellular level. Furthermore, lymphostin suppressed delayed-type hypersensitivity in mice. However, the inhibitory activity against Lck at the cellular level was weaker than that against the mixed lymphocyte reaction. Thus, we examined the effects of lymphostin on other kinases. Interestingly, lymphostin also inhibited phosphatidylinositol 3-kinase (IC_<50> 0.001 μM). Consequently, we conclude that lymphostin inhibits the mixed lymphocyte reaction and delayed-type hypersensitivity not only through the blockade of Lck, but through the blockade of phosphatidylinositol 3-kinase as well.
- 社団法人日本農芸化学会の論文
- 2002-03-23
著者
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NAKANISHI SATOSHI
Tokyo Research Laboratories, kyowa Hakko Kogyo Co., Ltd
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TANAKA Takeo
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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YANO Hiroshi
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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NAGATA Hiroyuki
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd
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TAKAHASHI Isami
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd
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TAMAOKI Tatsuya
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd
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Tamaoki T
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Nagata Hiroyuki
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Takahashi Isami
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Tanaka T
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Sato Sanae
Dairy Science Laboratory Graduate School Of Agriculture Hokkaido University
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Nakanishi Satoshi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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SATO Soichiro
Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Sato S
Hokkaido Univ. Hokkaido Jpn
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Sato Soichiro
Pharmaceutical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Takahashi I
Japanese Red Cross Aichi Blood Center Aichi Jpn
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Nagata Hidetomo
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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SASAKI Kimihito
Pharmaceutical Research Institute, Kyowa Hakko Kogyo Co., Ltd.
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Sasaki Kimihito
Pharmaceutical Research Institute Kyowa Hakko Kogyo Co. Ltd.
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Yano Hiroshi
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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SATO Soichiro
Pharmaceutical Research Institute, Kyowa Hakko Kogyo Co., Ltd.
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