Purification, characterization and sequence analyses of the extracellular giant hemoglobin from Oligobrachia mashikoi
スポンサーリンク
概要
- 論文の詳細を見る
We purified an extracellular hemoglobin with the molecular mass of ca. 440 kDa from the whole homogenates of Oligobrachia mashikoi (phylum Pogonophora) by a one-step gel-filtration. The preparation was pure to be crystallized. The P50 values of the hemoglobin and the fresh blood prepared from O. mashikoi were about 0.82 Torr and 0.9 Torr, respectively, which were much lower than the P50 value of human hemoglobin. However, the n values of the hemoglobin and the blood were about 1.2 and 1.1, respectively. Using the improved tricine SDS-PAGE, we could separate O. mashikoi hemoglobin into four kinds of the globin chains, A1, A2, B1 and B2, and succeeded for the first time in cloning and sequencing of the complete cDNA encoding B1 globin gene, in addition to A1, A2 and B2 globin genes in full length. We found that all globin genes have the extracellular signal sequences in each molecule and the distal His of the B1 globin chain is replaced to Gln. Finally, we constructed phylogenetic trees of the hemoglobins from Pogonophora, Vestimentifera and Annelida. © 2005 Zoological Society of Japan.
- 社団法人日本動物学会の論文
- 2005-03-25
著者
-
大浜 栄作
鳥取大学脳神経病理
-
大浜 栄作
鳥取大学医学部脳幹性疾患研究施設脳神経病理部門
-
大浜 栄作
鳥取大学医学部脳研脳神経病理部門
-
大浜 栄作
鳥取大学医学部附属病院 放射線科
-
大浜 栄作
鳥取大学医学部脳研脳神経病理
-
Sasayama Yuichi
Noto Marine Laboratory Institute Of Nature And Environment Technology Kanazawa University
-
Sasayama Yuichi
Noto Marine Laboratory Faculty Of Science Kanazawa University
-
Sasayama Yuichi
Institute Of Nature And Environmental Technology Kanazawa University
-
Sasayama Yuichi
Kanazawa Univ. Ishikawa Jpn
-
金森 正明
Department Of Life Science Graduate School Of Natural Science And Technology Kanazawa University
-
FUKUMORI YOSHIHIRO
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
-
Nakagawa Tomomi
Graduate School Of Life And Environmental Sciences University Of Tsukuba Tsukuba
-
KANEMORI MASAAKI
Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University
-
Nakagawa Taro
Department of Biology, Faculty of Science, Kanazawa University
-
Fukumori Yoshihiro
Kanazawa Univ. Kanazawa Jpn
-
Fukumori Yoshihiro
Department Of Biology Faculty Of Science Osaka University
-
Onoda Seiko
Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University
-
大浜 栄作
Noto Marine Laboratory Institute Of Nature And Environmental Technology Kanazawa University
-
大浜 栄作
鳥取大学医学部附属脳幹性疾患研究施設
-
Fukumori Y
Tokyo Inst. Technology Kanagawa Jpn
-
Sasayama Yuichi
Noto Marine Laboratory Division Of Biodiversity Institute Of Nature And Environmental Technology Kan
-
Onoda Seiko
Department Of Life Science Graduate School Of Natural Science And Technology Kanazawa University
-
Yajima Shinya
Department Of Biology Faculty Of Science Shizuoka University
-
Kanemori Masaaki
Department Of Life Science Graduate School Of Natural Science And Technology Kanazawa University
-
Fukumori Yoshihiro
Department Of Biology Faculty Of Science Kanazawa University
-
Nakagawa Taro
Department of Life Science, Graduate School of Natural Science and Technology, Kanazawa University
関連論文
- O2-24 周産期脳障害による多小脳回を有する難治性後頭葉てんかんの男性例(経過・予後2,一般演題(口演),第42回日本てんかん学会)
- 繰り返す意識障害と進行性痴呆を呈したspongiform leukoencephalopathyの1剖検例
- 小脳に多数のKuru斑を認めたCJDの1剖検例
- 全脳型Creutzfeldt-Jakob病におけるプリオン蛋白沈着に関する免疫組織化学的検討
- 急性脳症におけるアストロサイトのclasmatodendrosis
- 筋萎縮性側索硬化症の脊髄におけるHGF-cMet systemの発現に関する研究
- Advanced glycation endprodusts(AGEs)修飾superoxide dismutase 1(SOD1)凝集産物は家族性筋萎縮性側索硬化症(FALS)とそのモデルマウス(G85R transgenic mice)に共通する細胞傷害機序の一要因である
- 家族性筋萎縮性側索硬化症に出現するAstrocytic Hyaline Inclusions (Ast-HIs) におけるadvanced glycation endproduct (AGE) の局在意義 : 免疫組織化学的及び免疫電顕的解析
- 家族性筋萎縮性側索硬化症におけるAstrocytic Hyaline Inclusions(Ast-HIs)免疫組織化学的, 電顕的, 免疫電顕的検討
- 家族性筋萎縮性側索硬化症の長期生存2例に出現したglial inclusion : 免疫組織化学的及び電顕的研究
- 6-formylpterinのラット網膜における遅発性神経細胞死阻止効果 : 形態学的および免疫組織化学的研究
- EXPRESSION OF A- AND E-SUBUNITS OF VACUOLAR TYPE H^+ -ATPASE IN THE ENDOLYMPHATIC SAC OF THE BULLFROG(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- GENE EXPRESSION OF THE PROHORMONE CONVERTASES PC1 AND PC2 IN THE ULTIMOBRANCHIAL GLAND OF THE BULLFROG
- 脊髄皮質脊髄路の異形成を示した18トリソミー症候群の2剖検例
- ダウン症候群脳におけるBACE2発現の発達・加齢変化
- 広範な脳動脈解離をみとめた若年性脳梗塞の1例
- 再発型EAEに対するmizoribineの抑制効果 : 末梢血, リンパ節でのリンパ球表面抗原解析
- 高度な壊死性変化を示した進行性多巣性白質脳症(PML)の1剖検例
- 3)脊髄空洞症の病理
- SOD1遺伝子異常を伴うFALSとトランスジェニックラット(H46R・G93A)とにおけるSOD1凝集とレドックス制御システムとの関連について
- SOD1 遺伝子異常を伴う家族性筋萎縮性側索硬化症と G85R transgenic mouse とに共通して認められる granule-coated fibril 形成の分子生物学的意義
- ヒト胎児及び新生児脳の発達における SOD 及び NOS : 出生前後における両酵素の発現意義
- 全脳型Creutzfeldt-Jakob病の白質アストロサイトにおけるiNOSの発現 : 免疫組織化学的及び電顕的解析
- 脊髄空洞症
- Myxopapillary ependymomaと脊髄終糸の組織学的検討
- 脳梗塞における抗酸化ストレス蛋白質MSP23の発現
- O1-07 てんかん原性皮質異形成とヒト胎児脳におけるFGF-2の発現 : 免疫組織化学的定量解析(実験てんかん2,一般演題(口演),てんかん制圧:新たなステージに向けて,第41回日本てんかん学会)
- 老犬の脳における老人斑と脳血管アミロイド症の位置的関係
- Guglielmi detachable coils (GDCs) で治療された脳動脈瘤の瘤内血栓化の評価
- EP-1 選択的扁桃核海馬切除術によって治療された内側側頭葉てんかん患者における海馬および扁桃核の病理学的特徴(てんかん病理,第42回日本てんかん学会)
- Response of osteoblasts and osteoclasts in regenerating scales to gravity loading
- ALS運動ニューロンの細胞病理 (特集 神経病理:最前線)
- もやもや病の脳血管病変 : Moyamoya angiopathy
- 摂食障害・IVH管理後に発症した亜急性脊髄連合変性症の1剖検例
- Spinocerebellar ataxia type 7 (SCA7) の 1 剖検例
- 痴呆を伴う筋萎縮性側索硬化症 : 5例における臨床病理学的スペクトラムについて
- 家族性筋萎縮性側索硬化症:変異SOD1タンパク質の沈着 (特集 神経変性疾患 ニューロンの変性・死の普遍的メカニズム)
- オリーブ橋小脳萎縮症における小脳Purkinje細胞 : 形態計測およびIP_3R/P_400免疫組織化学的研究
- 実験的自己免疫性脳脊髄炎に合併する虹彩炎におけるストレス応答蛋白質60の発現 : 免疫組織化学的研究
- 緩徐進行性失語を呈した進行性核上性麻痺の一剖検例
- 歯状核赤核・淡蒼球ルイ体萎縮症 : CAGリピート数と病理形態所見
- 成人型正染性白質シストロフィーの1剖検例
- 経過中に振戦,舞踏様運動を呈した脊髄性進行性筋萎縮症の1剖検例
- アトピー白内障における水晶体上皮細胞障害とアポトーシス : 病理組織学的および免疫組織化学的研究
- 著明な Verocay body の形成を伴った皮下神経鞘腫の 1 例
- アストロサイト(病態) (特集 グリア細胞とその病態)
- Leukoencephalopathy with Vanishing White Matterの一剖検例
- ヒト小脳プルキンエ細胞の発達 : 免疫組織化学的検討
- Minigemistocyteに関する免疫組織化学的研究
- 脊椎・脊髄の解剖と病理
- 老年期に発症したレビー小体型痴呆純粋型の一剖検例
- 2.良性腫瘍の病理診断(LS 7 脳腫瘍の病理診断)
- Spinocerebellar ataxia type 2 (SCA2) の 1 剖検例
- 広範な大脳皮質及び基底核の神経細胞脱落を呈したPostconvulsive Brain Damageの1剖検例
- アンモン角形成異常を示したKallmann's Syndromeの2剖検例
- Familial juvenile parkinsonism: clinical and pathologic study in afamily
- POSSIBLE HORMONAL REGULATION ON DISSOLUTION OF CALCIUM CARBONATE CRYSTALS IN THE BULLFROG ENDOLYMPHATIC SAC
- Cloning and expression of vacuolar proton-pumping ATPase subunits in the follicular epithelium of the bullfrog endolymphatic sac
- 歯状核赤核・淡蒼球ルイ体萎縮症 : 親子剖検例における病変の強弱と拡がりに関する考察
- INVOLVEMENT OF VACUOLAR-H^+-ATPASE IN DISSOLVING BICARBONATE CRYSTALS LOCATED IN THE FOLLICLES OF THE ENDOLYMAPHIC SAC IN THE BULLFROG, RANA CATESBEIANA
- THE BEARD WORM (OLIGOBRACHIA MASHIKOI) ALSO PROTRUDE THE TENTACLES, WHEN HYDROGEN SULFIDE WAS SUPPLIED(Taxonomy and Systematics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Distribution and Population of Free-Living Cells Related to Endosymbiont A Harbored in Oligobrachia mashikoi (a Siboglinid Polychaete) Inhabiting Tsukumo Bay
- PA-53 Search for the genes related to chemoautotrophic sulfur oxidation in the genome of Oligobrachia mashikoi endosymbiont(Taxonomy/Phylogeny,Poster presentation A)
- Identification of Endosymbionts in Oligobrachia mashikoi (Siboglinidae, Annelida)
- CROSS TALK BETWEEN HOST CELLS AND SYMBIONTS OF THE BEARD WORM, OLIGOBRACHI MASHIKOI, POGONOPHORA : DISCUSSION FROM A CYTOARCHITECTURAL VIEWPOINT(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Soci
- VISUALIZATION OF BACTERIOCYTES IN THE TROPHOSOME OF THE BEARD WORM, OLIGOBRACHIA MASHIKOI(Taxonomy and Systematics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- A STUDY OF HISTOCHEMISTRY AND ULTRA-STRUCTURE OF THE TROPHOSOME IN THE BEARD WORM (OLIGOBRACHIA MASHIKOI)(Taxonomy and Systematics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- VARIATION ON MORPHOLOGY OF TENTACLES OF THE BEARD WORM, POGONOPHORA(OLIGOBRACHIA MASHIKOI)(Taxonomy and Systematics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- HISTOCHEMICAL STUDY OF THE TROPHOSOME OF THE BEARD WORM, POGONOPHORA (OLIGOBRACHIA MASHIKOI)(Taxonomy and Systematics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- MORPHOLOGICAL, PHYSIOLOGICAL AND ECOLOGICAL STUDY OF THE BEARD WORM, POGONOPHORA (OLIGOBRACHIA MASHIKOI)(Taxonomy and Systematics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- External Morphology of the Posterior End, the "Opisthosoma", of the Beard Worm Oligobrachia mashikoi (Pogonophora)
- PHYSIOLOGICAL ROLE OF CALCITONIN IN TELEOST REPRODUCTION
- PLASMA CALCITONIN AND CA LEVELS IN CHICKS SUFFERED FROM BENDING OF VERTEBRAE BY PINEALECTOMY
- PRIMARY STRUCTURE OF N-TERMINUS OF PROCALCITONIN (N-PROCT) IN SOME VERTEBRATES
- AMINO ACID SEQUENCE AND HYPOCALCEMIC POTENCY OF BULLFROG CALCITONIN
- Effects of Ultimobranchialectomy on the Mineral Balances of the Plasma and Bile in the Stingray (Elasmobranchii)
- Partial Characterization of a Calcitonin-like Substance from the Brockmann Bodied of the medaka Oryzias latipes
- Amino Acid Sequence of Sardine Calcitonin and Its Hypocalcemic Activity in Rats
- VARIABLE AMPHIBIAN CALCITONIN GENES
- cDNA ANALYSIS OF THE HYPOCALCEMIC HORMONE IN SOME TELEOSTS
- Grayish spot on goldfish chest : An explanation
- The location and morphology of the ultimobranchial gland in medaka, Oryzias latipes
- A histological investigation of the maturation of the acorn worm, an inhabitant of the Sea of Japan, and a suggestion about the relationship between synchronized spawning/spermiation and the tidal level
- 家族性筋萎縮性側索硬化症の神経病理学的検討 : 1年半及び11年の臨床経過を有する同胞2例
- FORMATION AND DISSOLUTION OF CALCIUM CARBONATE CRYSTALS IN THE BULLFROG ENDOLYMPHATIC SAC
- IMMUNOCYTOCHEMICAL LOCALIZATION OF PROHORMONE CONVERTASES PC1 AND PC2 IN THE CALCITONIN/CGRP CELLS
- Purification, characterization and sequence analyses of the extracellular giant hemoglobin from Oligobrachia mashikoi
- Relationship between the lifestyle of a siboglinid (Pogonophoran) polychaete, Oligobrachia mashikoi, and the total sulfide and nitrogen levels in its habitat
- ECOLOGICAL STUDY OF THE BEARD WORM UNDER NATURAL CONDITIONS, POGONOPHORA (OLIGOBRACHIA MASHIKOI)(Taxonomy and Systematics,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Vitamin D metabolites affect serum calcium and phosphate in freshwater catfish, Heteropneustes fossilis
- REGULATION OF OTOCONIN-22 mRNA EXPRESSION IN THE BULLFROG ENDOLYMPHATIC SAC BY CALCITONIN(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ENDOCRINE REGULATION OF EXPRESSION OF OTOCONIN-22 mRNA IN THE ENDOLYMPHATIC SAC OF THE BULLFROG, Rana catesbeiana(Endocrinology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Anuran calcitonins are diverse in lower vertebrates
- 淡蒼球黒質ルイ体萎縮症 : 組織学的, 免疫組織化学的検討
- A study of the sex ratio and fin morphometry of the Thai medaka, Oryzias minutillus, inhabiting suburbs of Bangkok, Thailand
- Nucleotide sequences of reptile calcitonins: Their high homology to chicken calcitonin
- PARTIAL SEQUENCE OF cDNA OF THE HYPOCALCEMIC HORMONE (CALCITONIN) IN THE NEWT
- SURVEY OF THE CALCITONIN GENES FROM TWO KINDS OF PRIMITIVE BONY FISHES : POLYPTERUS SENEGALUS ; ACIPENSER DABRYANUS
- Reproductive strategy of the tiny abyssal scallop (Delectopecten vitreus macrocheiricolus) collected on the bottom of the Japan Sea, surmised from histological observations of the gonads.
- Molecular Characterization and In Situ Localization of Endosymbiotic 16S Ribosomal RNA and RuBisCO Genes in the Pogonophoran Tissue