Carbohydrate Binding Specificity of the Recombinant Chitin-binding Domain of Human Macrophage Chitinase(Biochemistry & Molecular Biology)

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概要

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• The chitin-binding domain of human macrophage chitinase was expressed as a fusion protein with glutathione S-transferase in Escherichia coli and assayed for its binding activity. The purified recombinant chitin-binding domain bound to chitin, but not to glucan, xylan, or mannan. The binding of the recombinant chitin-binding domain to chitin was inhibited by N-acetylglucosamine, di-N-acetylchitobiose, and hyaluronan, but not by N-acetylgalactosamine or chondroitin. Furthermore, a solid-phase binding assay showed that the recombinant domain interacts specifically with hyaluronan and hybrid-type N-linked oligosaccharide chains on glycoproteins, and that the oligosaccharide-binding characteristics are similar to those of wheat germ agglutinin, a lectin that binds to chitin. The results suggest that human chitinase chitin-binding domain may be involved in tissue remodeling through binding to polysaccharides or extracellular matrix glycoproteins, and this recombinant protein can be used to elucidate biological functions of the enzyme.

• 社団法人日本農芸化学会の論文
• 2003-11-23

著者

• UJITA Minoru

  Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu

• HARA Akira

  Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu

• Hara Akira

  First Department Of Internal Medicine. Fukui Medical School

• Sakai Kaori

  名城大学 農

• Ujita M

  Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University

• Hara A

  First Department Of Internal Medicine. Fukui Medical School

• Hirakawa Akina

  From The Third Division Department Of Internal Medicine Kyoto University

• Hara A

  Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University

• Hara Akira

  Shonan Institute Of Technology

• Yoneda Masahiko

  Aichi Prefectural Collage of Nursing and Health

• Isomura Shigeki

  Agricultural High-Tech Research Center, Meijo University

• SAKAI Kaori

  Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu

• HAMAZAKI Keishi

  Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu

• ISOMURA Shigeki

  Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Agricultural High-Te

• Hirakawa Akiha

  3rd Dept. Of Intern. Med. Kyoto Univ.

• Sakai Kaori

  Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture

• Isomura Shigeki

  Agricultural High-tech Research Center Meijo University

• Hamazaki Keishi

  Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture

• Ujita Minoru

  Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture

• Hara Akira

  Laboratory Of Biochemistry Gifu Pharmaceutical University

• Hara Akira

  From The Third Division Department Of Internal Medicine Kyoto University

• Hirakawa Akina

  3rd Medical Clinic Faculty Of Medicine Kyoto University

• Yoneda Masahiko

  Aichi Prefectural College Of Nursing And Health

• Hara Akira

  Laboratory Of Biochemistry And Nutritional Chemistry:(present Address)faculty Of Agriculture Meijo U

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