Binding Specificity of the Recombinant Cytoplasmic Domain of Cordyceps militaris β-1,3-Glucan Synthase Catalytic Subunit
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概要
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The cytoplasmic domain of the medicinal mushroom Cordyceps militaris β-1,3-glucan synthase catalytic subunit Fks1 was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and was assayed for binding specificity. The recombinant cytoplasmic domain bound specifically to UDP-agarose and lichenan (β-glucan), but not to ADP-agarose, GDP-agarose, or other carbohydrates.
著者
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UJITA Minoru
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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KATSUNO Yosuke
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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INOUE Ryosuke
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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MAKINO Yusuke
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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OKUMURA Hiroki
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Katsuno Yosuke
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Ujita Minoru
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Okumura Hiroki
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Makino Yusuke
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Inoue Ryosuke
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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