Carbohydrate Binding Specificity of Recombinant Human Macrophage β-Glucan Receptor Dectin-1
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概要
- 論文の詳細を見る
Human macrophage dectin-1, a type II transmembrane β-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and assayed for binding specificity. Recombinant dectin-1 specifically bound to some β-glucans, but not to other carbohydrates. The β-glucan binding of recombinant dectin-1 was inhibited by laminarin, a soluble β-glucan, and by laminarioligosaccharides, but not by other carbohydrates. These results suggest that recombinant human dectin-1 can be used as a useful probe in identifying ligands in humans and tonic foods due to its strict binding specificity.
- 社団法人 日本農芸化学会の論文
- 2009-01-23
著者
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UJITA Minoru
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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OKUMURA Hiroki
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Nagayama Hiroko
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Kanie Satoko
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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KOIKE Shota
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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IKEYAMA Yoshiko
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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OZAKI Takahiro
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Faculty of Agricultu
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Ujita Minoru
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Ujita Minoru
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Koike Shota
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Nagayama Hiroko
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Ozaki Takahiro
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Okumura Hiroki
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Ikeyama Yoshiko
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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Kanie Satoko
Laboratory Of Biological Chemistry Department Of Applied Biological Chemistry Faculty Of Agriculture
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