Characterization of Styrene Oxide Isomerase, a Key Enzyme of Styrene and Styrene Oxide Metabolism in Corynebacterium sp.
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概要
- 論文の詳細を見る
Styrene oxide isomerase (SOI) [EC 5.3.99.7], most probably located in the cell wall, was partially purified from Corynebacterium sp. AC-5 cells grown in a styrene gas atmospheres. The enzyme catalyzed the isomerization reaction to give phenylacetaldehyde, but did not catalyze its reverse reaction. The optimum pH of the reaction was around 7.0, and the enzyme was unstable below pH 6.0. The K_m toward styrene oxide was very low (7.7×10^<-5> M), indicating its high affinity for styrene oxide. The enzyme showed strict substrate specificity, and epoxide compounds other than styrene oxide did not serve as substrates. (S)-Styrene oxide was preferentially converted by the enzyme, compared with the (R)-isomer. The possible application of SOI as a biocatalyst is also discussed.
- 社団法人日本農芸化学会の論文
- 1997-12-23
著者
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Itoh Nobuya
Biotechnology Research Center Toyama Prefectural University
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ITO Takeshi
Department of Ophthalmology and Visual Science, Nagoya City University Graduate School of Medical Sc
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OKADA Keisaku
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
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HAYASHI Kunimasa
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
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MIZUGUCHI Naoyuki
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
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Okada Keisaku
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
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Hayashi Kunimasa
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
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Mizuguchi Naoyuki
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
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Ito Takeshi
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
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Ito Takeshi
Department Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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