Efficient Transformation of Pseudomonas Strains with pNI Vectors by Electroporation
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概要
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The optimum conditions for electro-transformation of some Pseudomonas strains including P. putida, P. fluorescens, and P. flavida, were detined using the pNI105 vector, resulting in 100-10,000 fold increases in transformation efficiency compared with conven tional chemical transformation with MgCl_2. The growth phase of the cultured cells and the tield strength were important in obtaining high transformation efficiency. Under optimal conditions, 3. 2 × 10^7transformants per 1μg DNA were obtained using a combination of pNI105 and P. fluorescens IAM12022. This value is the highest ever obtained for a Pseudomanas species. In addition, the procedures were applicable to the effective transformation of fluorescent Pseudomonas strains with pNI vectors.
- 社団法人日本農芸化学会の論文
- 1994-07-23
著者
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Itoh Nobuya
Department of Biotechnology, Faculty of Engineering (Biotechnology Research Center), Toyama Prefectu
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Itoh N
Biotechnology Research Center Toyama Prefectural University
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Itoh Nobuya
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
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Koide Yoshinao
Research Laboratory Amano Pharmaceutical Co. Ltd.
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Kouzai Toyoko
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
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Kouzai Toyoko
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
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ITOH Nobuya
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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