Isolation and characterization of styrene metabolism genes from styrene-assimilating soil bacteria Rhodococcus sp. ST-5 and ST-10(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Styrene metabolism genes were isolated from styrene-assimilating bacteria Rhodococcus sp. ST-5 and ST-10. Strain ST-5 had a gene cluster containing four open reading frames which encoded styrene degradation enzymes. The genes showed high similarity to styABCD of Pseudomonas sp. Y2. On the other hand, strain ST-10 had only two genes which encoded styrene monooxygenase and flavin oxidoreductase (styAB). Escherichia coif transformants possessing the sty genes of strains ST-5 and ST-10 produced (S)-styrene oxide from styrene, indicating that these genes function as styrene degradation enzymes. Metabolite analysis by resting-cell reaction with gas chromatography-mass spectrometry revealed that strain ST-5 converts styrene to phenylacetaldehyde via styrene oxide by styrene oxide isomerase (styC) reaction. On the other hand, strain ST-10 lacked this enzyme, and thus accumulated styrene oxide as an intermediate. HPLC analysis showed that styrene oxide was spontaneously isomerized to phenylacetaldehyde by chemical reaction. The produced phenylacetaldehyde was converted to phenylacetic acid (PAA) in strain ST-10 as well as in strain ST-5. Furthermore, phenylacetic acid was converted to phenylacetyl-CoA by the catalysis of phenylacetate-CoA ligase in strains ST-5 and ST-10. This study proposes possible styrene metabolism pathways in Rhodococcus sp. strains ST-5 and ST-10.
著者
-
Itoh Nobuya
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Fukui University
-
Toda Hiroshi
Department Of Anesthesia Kyoto University Hospital
-
Itoh Nobuya
Department Of Biotechnology Faculty Of Engineering Biotechnology Research Center Toyama Prefectural
-
Toda Hiroshi
Department Of Biotechnology Faculty Of Engineering Biotechnology Research Center Toyama Prefectural
-
ITOH Nobuya
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
関連論文
- Gene cloning and characterization of dihydrolipoamide dehydrogenase from Microbacterium luteolum : A useful enzymatic regeneration system of NAD^+ from NADH(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 3P-006 蛋白質のデコイ構造識別関数DFMACの拡張と性能評価(蛋白質-構造,第47回日本生物物理学会年会)
- Genetic Alterations of Mixed Hyperplastic Adenomatous Polyps in the Colon and Rectum
- Gene Cloning of an Endoglucanase from the Basidiomycete Irpex lacteus and Its cDNA Expression in Saccharomyces cerevisiae
- Vasodilative Effect of Adrenomedullin in Isolated Arteries of the Dog
- Interactions between morphine and nitric oxide in various organs
- Anesthetic modulation of immune reactions mediated by nitric oxide
- Efficient Transformation of Pseudomonas Strains with pNI Vectors by Electroporation
- Gene Cloning of Cellobiohydrolase II from the White Rot Fungus Irpex lacteus MC-2 and Its Expression in Pichia pastoris
- Accumulation of Tryptamine in Barley Leaves Irradiated with UV Light
- Isolation and Identification of Styrene-degrading Corynebacterium Strains, and Their Styrene Metabolism
- A Novel Immunohistochemical Staining Method Allows Ultrarapid Detection of Lymph Node Micrometastases While Conserving Antibody
- Isolation and characterization of styrene metabolism genes from styrene-assimilating soil bacteria Rhodococcus sp. ST-5 and ST-10(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- The thermal decomposition of lithium hydrogenphosphoramidate trihydrate.
- Halothane-induced relaxation of vascular smooth muscle: A possible contribution of increased cyclic GMP formation.