A Novel Immunohistochemical Staining Method Allows Ultrarapid Detection of Lymph Node Micrometastases While Conserving Antibody
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概要
- 論文の詳細を見る
We developed a novel ultrarapid immunohistochemical staining method in which an AC electric field is used to facilitate detection of tumor cells. Frozen sections of non-small cell lung cancer in lymph nodes were fixed in acetone for 2 min, after which they were incubated for 2 min with an anti-pancytokeratin antibody cocktail and then with EnVisionTM complex under an alternating current (AC) electric field. The sections were then incubated with a chromogen (3,3diaminobenzidine) for 3 min and counterstained with hematoxylin. This method enabled detection of tumor cells in frozen sections in less than 15 min. In addition, we were able to reduce the amount of antibody used by more than 90% when the sections were incubated under the AC electric field for a longer period. This method could be a useful tool for frozen section diagnosis and research. Furthermore, with this method the cost of immunohistochemical staining can be reduced.
著者
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Nanjo Hiroshi
Division Of Clinical Pathology Akita University Graduate School Of Medicine
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Toda Hiroshi
Department Of Anesthesia Kyoto University Hospital
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Saito Hajime
Department Of Biology Faculty Of Science Okayama University
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Konno Hayato
Department of Chest Surgery, Akita University Hospital and Akita University Graduate School of Medicine
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Minamiya Yoshihiro
Department of Chest Surgery, Akita University Hospital and Akita University Graduate School of Medicine
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Motoyama Satoru
Department of Chest Surgery, Akita University Hospital and Akita University Graduate School of Medicine
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ITO Manabu
Department of Advanced Medicine for Spine and Spinal Cord Disorders, Hokkaido University Graduate School of Medicine
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Kagaya Masami
Akita Prefectural Research and Development Center
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OGAWA Junichi
Department of Internal Medicine, Kawasaki City Hospital
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Ogawa Junichi
Department of Thoracic Surgery, Akita University Graduate School of Medicine
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Motoyama Satoru
Department of Thoracic Surgery, Akita University Graduate School of Medicine
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Akagami Yoichi
Akita Prefectural Research and Development Center
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Saito Hajime
Department of Thoracic Surgery, Akita University Graduate School of Medicine
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Saito Hajime
Department of Applied Chemistry, Faculty of Engineering, Nagoya University
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