The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61

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Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP_6). The pathway of hydrolysis of InsP_6 by phytase from wheat bran of Triticum aestivum L. cv. Nourin #61 is proved in this study. Structures of the intermediates were established by a varlety of nuclear magnetic resonance techniques (^1H-, two-dimensional ^1H-^1H coupling-correlation spectra and two-dimensional ^<31>P-^1H correlation spectra), gas chromatography, and bioassay. On the basis of the structures identified, initial hydrolysis of the phosphate ester occurs at the D/L-4 position of InsP_6 to yield D/L-Ins (1,2,3,5,6) P_5. After the dephosphorylation, the pathway of dephosphorylation is divided into two routes. The main route proceeds via D/L-Ins (1,2,5,6) P_4,D/L-Ins (1,2,6) P_3 and D/L-Ins (1,2) P_2,while the minor route proceeds via D/L-Ins (1,2,3,6) P_4,Ins (1,2,3) P_3 and D/L-Ins (1,2) P_2. D/L-Ins (1,2) P_2 is hydrolyzed at the D/L-1 or 2-position, and finally myo-inositol is produced.
社団法人日本農芸化学会の論文
2000-05-23