Gene Expression during P_i Deficiency in Pholiota nameko : Accumulation of mRNAs for Two Transporters(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
The effects of P_i deficiency on gene expression in Pholiota nameko were examined. A cDNA library was constructed from poly(A)^+ RNA isolated from mycelia cultured in P_i-depleted (P^-) media, and 150 clones corresponding to P_i deficiency-inducible (pdi) genes were selected by differential hybridization with probes prepared from poly(A)^+ RNAs from the mycelia cultured in the P_i-supplied (P^+) and P^- media. These clones were considered to derive from 31 genes by cross-hybridization. Northern blot analysis showed that these pdi genes were expressed in various patterns during P_i deficiency. Among the clones, the DNA sequences of pdi85 and pdi343 were analyzed. The deduced amino acid sequences indicated that they have structural similarities to P_i and metabolite transporters.
- 社団法人日本農芸化学会の論文
- 2002-04-23
著者
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Hara Takeshi
Department Of Intelligent Image Information Division Of Regeneration And Advanced Medical Sciences G
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Hara Takeshi
Graduate School Of Medicine Gifu University
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Joh Toshio
Faculty Of Agriculture Niigata University
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Hara T
Department Of Intelligent Image Information Division Of Regeneration And Advanced Medical Sciences G
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TASAKI Yuji
Graduate School of Science and Technology, Niigata University
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Tasaki Yuji
Graduate School Of Science And Technology Niigata University
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HARA Takashi
Faculty of Engineering,Gifu University, Gifu-shi
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KAMIYA Yuki
Faculty of Agriculture, Niigata University
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AZWAN A.
Graduate School of Science and Technology, Niigata University
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Azwan A.
Graduate School Of Science And Technology Niigata University
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Kamiya Yuki
Faculty Of Agriculture Niigata University
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Hara Takashi
Faculty Of Agriculture Niigata University
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