Mouse Epidermal Keratinocytes in Three-Dimensional Organotypic Coculture with Dermal Fibroblasts Form a Stratified Sheet Resembling Skin

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概要

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• We describe an organotypic model of mouse skin consisting of a stratified sheet of epidermal keratinocytes and dermal fibroblasts within a contracted collagen gel. The model was designed to maintain the polarity of stratified keratinocytes and permit their long-term culture at an air-liquid interface. After air exposure, the thickness of the keratinocyte sheet transiently increased and then decreased to two cell layers at 2 weeks. The two-cell-layer structure is similar to that of the adult mouse epidermis. Cytokeratin 5 was localized in the lowest cell layer in the epithelial sheet, but cytokeratin 1 and loricrin were localized in the outer cell layers, resembling mouse skin. The expressions of interleukin 1α and 1β in the keratinocytes and of keratinocyte growth factor 1 and 2 in the fibroblasts correlated with keratinocyte stratification. The mouse organotypic coculture is useful in studying epithelial cell-mesenchymal cell interactions in vitro.

• 社団法人 日本農芸化学会の論文
• 2006-11-23

著者

• Hara Takeshi

  Department Of Intelligent Image Information Division Of Regeneration And Advanced Medical Sciences G

• Saito Yuriko

  Department Of Animal Resource Sciences Graduate School Of Agricultural And Life Sciences The Univers

• Chida Kazuhiro

  Department Of Animal Resource Sciences Graduate School Of Agricultural And Life Sciences The Univers

• IKUTA Shuzo

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• SEKINO Nobufumi

  Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The Unive

• Ikuta Shuzo

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Sekino Nobufumi

  Department Of Animal Resource Sciences Graduate School Of Agricultural And Life Sciences The Univers

• Hara Takeshi

  Department Of Applied Chemistry Kyushu Institute Of Technology

• Hara Takeshi

  Department Of Animal Resource Sciences Graduate School Of Agricultural And Life Sciences The Univers

• CHIDA Kazuhiro

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

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