Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61
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概要
- 論文の詳細を見る
Two phytase isozymes (PHY1 and PHY2) were purified homogeneously from bran of Triticum aestivum L. cv. Nourin #61 by (NH4)2SO4 fractionation, methanol fractionation, Sephacryl S-200 HR gel filtration chromatography, DEAE-TOYOPEARL chromatography, CM-TOYOPEARL chromatography and second Sephacryl S-200 HR gel filtration chromatography. Molecular weights of the two enzymes were 71,000 and 66,000 by gel filtration, and 68,000 and 66,000 by SDS-PAGE, respectively. Optimum pH and temperatures were 6.0 and 45℃ for PHY1, and 5.5 and 50℃ for PHY2. The activity of both phytases was stable at pHs between pH 4.0-7.0 and below 40℃. The Km values for myo-inositol hexakisphosphate (IHP) were 0.48 μM for PHY1 and 0.77 μM for PHY2. The Ki values for Pi were 2.69 mM for PHY1 and 6.59 mM for PHY2. Both phytases showed relatively high specificity for IHP.
- 社団法人 日本食品科学工学会の論文
- 1999-02-01
著者
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Nakano Tomoki
Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University
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Nakano T
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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JOH Toshio
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Joh Toshio
Faculty Of Agriculture Niigata University
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Joh Toshio
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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HAYAKAWA Toshiro
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Hayakawa T
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Hayakawa Toshiro
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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NAKANO Tadao
Graduate School of Science and Technology, Niigata University
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TOKUMOTO Emi
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Tokumoto Emi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Nakano Toshiki
Marine Biochemistry Laboratory Gradate School Of Agricultural Science Tohoku University
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