Effect of Phytate on the Hydrolysis of p-Nitrophenyl Phosphate with Phosphatase from Various Sources(Food & Nutrition)
スポンサーリンク
概要
- 論文の詳細を見る
Phosphatase activity, using p-nitrophenyl phosphate(NPP) as the substrate, was inhibited by myo-inositol hexaphosphate(phytate). The extent of inhibition by 5 HIM phytate of the alkaline phosphatase activity of calf intestine and Escherichia coli, and of the acid phosphatase activity of wheat germ was 82, 56 and 50%, respectively. However, acid phosphatases from rice bran were not inhibited by 10mM phytate. The effect of preincubation with phytate on the acid and alkaline phosphatase activity was investigated. After a 180-min preincubation with 1 mM phytate, the extent of inhibition was 98% for the calf intestine enzyme, 88% for the E. coll enzyme, and 75% for the wheat germ enzyme. Mg^<2+> enhanced the phosphatase activity, but the addition of phytate lowered it. The inhibition of E. coli alkaline phosphatase by phytate was noncompetitive, with an apparent inhibitor constant of 0.45 HIM under the assay conditions. The inhibition of calf intestine alkaline phosphatase and of wheat germ acid phosphatase was of the mixed type, with inhibitor constants of 0.13niM and 0.43 μM, respectively. Therefore, phytate may interact with a complex of these phosphatases and substrates.
- 社団法人日本農芸化学会の論文
- 1991-03-23
著者
-
Sato Naomi
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University:(present Office)depar
-
Igaue Ikuo
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
-
Igaue Ikuo
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
-
HAYAKAWA Toshiro
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
-
Hayakawa Toshiro
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
-
OKADA Fusako
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
-
TSUTSUI Michiko
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
-
Okada Fusako
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University:(present Office)depar
-
Tsutsui Michiko
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University:(present Office)depar
-
HAYAKAWA Toshiro
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
関連論文
- Purification of β-Glucan Synthase II from Suspension-cultured Rice Cells
- Characterization and Solubilization of β-Glucan Synthases from Cultured Rice Cells
- Structure and Function of the Golgi Complex in Rice Cells : Characterization of Golgi Membrane Glycoproteins
- Localization of Binding Sites for Peanut, Ulex europaeus, and Castor Bean Lectin in the Rice Golgi Complex
- Preparation of Golgi Membrane Fractions Containing Lectin-binding Sites from Suspension-cultured Rice Cells
- Isolation and Characterization of Golgi Membranes from Suspension-cultured Cells of Rice (Oryza sativa L.)
- Isolation and Properties of Glucose-1-phosphatase from Mycelia of Pholiota nameko
- EPHA2/EFNA1 expression in human gastric cancer
- Purification of Phosphoinositol Kinase from Suspension-cultured Cells of Rice (Oryza sativa L.)
- Aggregated Forms of Glucose-6-phosphate Dehydrogenase from Cultured Rice Plant Cells
- A New Chemotype of Eubacterium saburreum : Isolation and Identification of L-threo-2-Pentulose from the Antigenic Polysacchande of E. saburreum T15
- The Pathway of Dephosphorylation of myo-Inositol Hexakisphosphate by Phytases from Wheat Bran of Triticum aestivum L. cv. Nourin #61
- Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61
- Myo-inositol Polyphosphate Intermediates in the Dephosphorylation of Phytic Acid by Acid Phosphatase with Phytase Activity from Rice Bran(Biological Chemistry)
- Effect of Phytate on the Hydrolysis of p-Nitrophenyl Phosphate with Phosphatase from Various Sources(Food & Nutrition)
- Induction and Secretion of RNA-degrading Enzymes by Phosphate Deficiency in Pholiota nameko(Biochemistry & Molecular Biology)
- Formation of a series of myo-inositol phosphates during growth of rice plant cells in suspension culture
- Purification and Characterization of Acid Phosphatases with or without Phytase Activity from Rice Bran(Biological Chemistry)
- Purification and Characterization of Phosphatidylinositol-specific Phospholipase C in Suspension-cultured Cells of Rice (Oryza sativa L.)
- Alcohol Dehydrogenase from Cultured Rice Cells
- Sugar Nucleotide Contents in Suspension-cultured Rice Cells(Biological Chemistry)
- Association between dopamine beta hydroxylase rs5320 polymorphism and smoking behaviour in elderly Japanese
- Association between neuropeptide Y receptor 2 polymorphism and the smoking behavior of elderly Japanese
- Characterization of Viscoelastic Properties of Rice Cake
- Characterization of Factors Affecting Properties of Rice Cake and Its Structure
- Occurrence of Organic Acids from myo-Inositol in Cultured Rice Cells