Alcohol Dehydrogenase from Cultured Rice Cells
スポンサーリンク
概要
- 論文の詳細を見る
Rice cells in suspension culture had high alcohol dehydrogenase activity during the logarithmic growth phase (3rd to 5th day). Ethanol was accumulated both in the cells and in the medium. The highest amount of ethanol was accumulated on the 4th day in cells (10μmoles/g fresh weight) and during the stationary growth phase (8th day) (180 m_M, ca. 1%) in the medium. The enzyme was isolated from the cell extract and purified 36-fold with a 14% yield by ammonium sulfate fractional precipitation, and chromatography on DEAE-Sephadex, Sephadex G-150 and Blue Dextran-Sepharose. The purified enzyme was homogeneous, as judged by its sedimentation velocity, and poly-acrylamide gel, starch gel and SDS-polyacrylamide gel electrophoreses. Its molecular weight was 76,000 distributed in two, identical 37,000 subunits. The isoelectric point was at pH 5.5. The enzyme contained 2.1 g atoms of zinc, 12 free SH groups and 3 to 4 SS bonds per molecule. The pH optimum for ethanol oxidatioa was pH 9.5 and for acetaldehyde reduction pH 6.0. The Km values for ethanol, NAD^+, acetaldehyde and NADH were 64.5 m_M, 47.1μM, 1.3 m_M and 9.5μM. The amino acid composition, substrate specificity, and the effects of chelators, SH reagents and sugar metabolic intermediates also are reported.
- 日本植物生理学会の論文
著者
-
Igaue Ikuo
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
-
Yagi Masahide
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
-
Yagi Masahide
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
関連論文
- Purification of β-Glucan Synthase II from Suspension-cultured Rice Cells
- Characterization and Solubilization of β-Glucan Synthases from Cultured Rice Cells
- Structure and Function of the Golgi Complex in Rice Cells : Characterization of Golgi Membrane Glycoproteins
- Localization of Binding Sites for Peanut, Ulex europaeus, and Castor Bean Lectin in the Rice Golgi Complex
- Preparation of Golgi Membrane Fractions Containing Lectin-binding Sites from Suspension-cultured Rice Cells
- Isolation and Characterization of Golgi Membranes from Suspension-cultured Cells of Rice (Oryza sativa L.)
- Aggregated Forms of Glucose-6-phosphate Dehydrogenase from Cultured Rice Plant Cells
- Myo-inositol Polyphosphate Intermediates in the Dephosphorylation of Phytic Acid by Acid Phosphatase with Phytase Activity from Rice Bran(Biological Chemistry)
- Effect of Phytate on the Hydrolysis of p-Nitrophenyl Phosphate with Phosphatase from Various Sources(Food & Nutrition)
- Formation of a series of myo-inositol phosphates during growth of rice plant cells in suspension culture
- Purification and Characterization of Acid Phosphatases with or without Phytase Activity from Rice Bran(Biological Chemistry)
- Purification and Characterization of Phosphatidylinositol-specific Phospholipase C in Suspension-cultured Cells of Rice (Oryza sativa L.)
- Alcohol Dehydrogenase from Cultured Rice Cells
- Sugar Nucleotide Contents in Suspension-cultured Rice Cells(Biological Chemistry)
- Occurrence of Organic Acids from myo-Inositol in Cultured Rice Cells