Characterization and Solubilization of β-Glucan Synthases from Cultured Rice Cells
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概要
- 論文の詳細を見る
Two types of β-glucan synthases, GS-I and GS-II, were found in cultured rice cells (Oryza sativa L.). In glycerol density gradient centrifugation, GS-I activity peak co-migrated with a marker enzyme of the Golgi membrane, while GS-II co-migrated with the plasma membrane. Analysis of the reaction products of GS-I and GS-II, suggested that GS-I and GS-II were mainly β-1,4,- and β-1,3-glucosyltransferases, respectively. GS-I had a higher substrate affinity for UDP-glucose than GS-II, and needed divalent cations for its activity. Effects of nucleotides on the activity were also considerably different between GS-I and GS-II. GS-I was solubilized well with CHAPS and digitonin, and GS-II was solubilized effectively with sucrose monolaurate.
- 社団法人日本農芸化学会の論文
- 1992-03-23
著者
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MORITA Tatsuya
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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Morita Tatsuya
静岡大学 農学部応用生物化学
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Morita Tatsuya
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Morita T
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Kuribayashi Ikuo
Department of Biosystem Science, Graduate School of Science and Technology, Faculty of Agriculture,
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Morita Takuya
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Igaue Ikuo
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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KIMURA Shinn
Department of Biosystem Science, Graduate School of Science and Technology (Niigata University)
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Igaue I
Niigata Univ. Niigata Jpn
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Igaue Ikuo
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Kimura Shinn
Department Of Biosystem Science Graduate School Of Science And Technology (niigata University)
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Kuribayashi Ikuo
Department Of Biosystem Science Graduate School Of Science And Technology Faculty Of Agriculture Nii
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