Myo-inositol Polyphosphate Intermediates in the Dephosphorylation of Phytic Acid by Acid Phosphatase with Phytase Activity from Rice Bran(Biological Chemistry)

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概要

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• Myo-inositol phosphate intermediates formed during dephosphorylation of phytic acid (by rice bran acid phosphatase with phytase activity F2) were isolated by ion-exchange chromatography. Hydrolysis of phytic acid proceeded via L-myo-inositol 1,2,3,4,5 and D-myo-inositol 1,2,4,5,6-pentakisphosphate, D-myo-inositol 1,2,5,6 and L-myo-inositol 1,2,3,4-tetrakisphosphate, D-myo-inositol 1,2,6 and myo-inositol 1,2,3-trisphosphate, D-myo-inositol 1,2-bisphosphate and finally myo-inositol monophosphate (myo-inositol 2,93%; D-myo-inositol 1,7%). Six percent of myo-inositol and 85% of Pi were found in the reaction mixture when the hydrolysis of phytic acid by the enzyme did not proceed further. The initial points of dephosphorylation of phytic acid by the phytase took place at L-myo-inositol 6(85%), D-myo-inositol 3(7%), myo-inositol 2(5%), and myo-inositol 5(3%) at the positions of the myo-inositol ring.

• 社団法人日本農芸化学会の論文
• 1990-02-23

著者

• SUZUKI Kayoko

  Department of Clinical Pharmacology and Toxicology, Showa Pharmaceutical University

• Igaue Ikuo

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Igaue Ikuo

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University

• Ohno Takao

  Analytical Instruments Laboratory Niigata University

• Suzuki Kayoko

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University:(present Office)mitsu

• Miura Hidehiko

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University:(present Office)eizai

• HAYAKAWA Toshiro

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Hayakawa Toshiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University

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