Specific Determination of Histamine in Fish by High-performance Liquid Chromatography after Diazo Coupling

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概要

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• A new approach to the pre-column derivatization and analysis of histamine by HPLC is described, the method being based upon the formation of a diazo-coupled derivative of histamine. This derivatization method is simple, efficient, sensitive and specific for histamine and other imidazole compounds without a preliminary clean-up step. The liquid chromatographic system allows for rapid, bonded-phase separation with visible-light detection of these compounds within 20 min at a 10-pmol sensitivity. By using this method, the production of a large amount of histamine was confirmed in the muscle of mackerel Scomber japonicus incubated with the intestinal contents of the mackerel.

• 1995-07-23

著者

• SATO Yoji

  Division of Biochemistry, Faculty of Fisheries, Nagasaki University

• Nakano Tomoki

  Department Of Biochemical Science And Technology Faculty Of Agriculture Kagoshima University

• Nakano T

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• Takeuchi Masaaki

  Marine Biochemistry Laboratory Graduate School Of Agricultural Science Tohoku University

• SATO Minoru

  Laboratory of Marine Biochemistry, Graduate School of Agricultural Science, Tohoku University

• NAGAHISA Eizou

  School of Fisheries Science, Kitasato University

• Sato M

  Graduate School Of Agricultural Science Tohoku University

• Kumagai T

  Tohoku Univ. Sendai Jpn

• Takeuchi M

  Laboratory Of Molecular And Cellular Biology Department Of Life Science Faculty Of Bioresources Mie

• SATO Minoru

  Faculty of Agriculture, Tohoku University

• TAKEUCHI Masaaki

  Faculty of Agriculture, Tohoku University

• Nakano Toshiki

  Faculty of Agriculture, Tohoku University

• Kumagai Tsutomu

  Faculty of Science, Tohoku University

• Kanno Nobuhiro

  School of Fisheries Sciences, Kitasato University

• Sato Yoshikazu

  School of Fisheries Sciences, Kitasato University

• Nobuhiro Kanno

  School Of Fisheries Sciences Kitasato University

• Nagahisa Eizou

  School Of Fisheries Sciences Kitasato University

• Sato Y

  Division Of Biochemistry Faculty Of Fisheries Nagasaki University

• Nakano Toshiki

  Marine Biochemistry Laboratory Gradate School Of Agricultural Science Tohoku University

• Kumagai Tsutomu

  Faculty Of Science Tohoku University

• Sato M

  Faculty Of Agriculture Tohoku University

• Sato Minoru

  Faculty Of Agriculture Tohoku University

• Takeuchi Masaaki

  Faculty Of Agriculture Tohoku University

• Kanno Nobuhiro

  School of Fisheries Science, Kitasato University

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