Molecular Characterization of the ZKT Gene Encoding a Protein with PDZ, K-Box, and TPR Motifs in Arabidopsis

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概要

• 論文の詳細を見る

• The ZKT gene from Arabidopsis encodes a polypeptide of 335 amino acid residues, with a calculated molecular mass of 37.4 kDa. ZKT is a member of a novel protein family present in the plant kingdom, which contains a PDZ, a K-box, and a TPR motif. A BLAST search indicated that the ZKT gene is a single gene in Arabidopsis and that ZKT homologs are present in soybean and rice but not in animals. The level of ZKT mRNA decreased after wounding. Antisera from rabbit immunized with recommbinant ZKT protein recognized a protein of 37 kDa in Arabidopsis. Western analysis with anti-ZKT antibody indicated that the level of ZKT protein does not change after wounding. The ZKT protein has consensus sequence motifs for phosphorylation. Immunoprecipitation with anti-ZKT antibody and western analysis with anti-phosphoamino acid antibody indicated that the ZKT protein is phosophorylated at the threonine and serine residues after wounding. These results suggest that the ZKT protein may act as a molecular adaptor regulated by phosphorylation in wound responses.

• 社団法人 日本農芸化学会の論文
• 2005-05-23

著者

• IWASAKI Yukimoto

  Department of Bioscience, Fukui Prefectural University

• TANAKA Hideaki

  Department of Gastroenterology and Hepatology, Division of Clinical Medicine and Biomedical Science,

• ISHIKAWA Atsushi

  Department of Bioscience, Fukui Prefectural University

• Ishikawa A

  Department Of Bioscience Fukui Prefectural University

• Ishikawa Atsushi

  Bio-polymer Research Co. Ltd. :(present Address)nakano Central Institute Nakano Vinegar Co. Ltd.

• Ishikawa Atsushi

  Department Of Bioscience Fukui Prefectural University

• KATO Chiyuki

  Department of Bioscience, Fukui Prefectural University

• ASAHI Tadashi

  Department of Bioscience, Fukui Prefectural University

• Asahi Tadashi

  Department Of Bioscience Fukui Prefectural University

• Asahi Tadashi

  Department Of Bioscience Faculty Of Biotechnology Fukui Prefectural University

• Kato Chiyuki

  Department Of Bioscience Fukui Prefectural University

• Iwasaki Yukimoto

  Department Of Bioscience Fukui Prefectural University

• Iwasaki Yukimoto

  Department Of Bioscience Faculty Of Biotechnology Fukui Prefectural University

• Tanaka Hideaki

  Department Of Biopharmaceutics Kyoto Pharmaceutical University

• Iwasaki Y

  Department Of Bioscience Fukui Prefectural University

• Ishikawa Atsushi

  Department Of Bioscience Faculty Of Biotechnology Fukui Prefectural University

• Ishikawa Atsushi

  Department Of Anatomy Fukuoka University School Of Medicine

• Tanaka Hideaki

  Department Of Bioscience Fukui Prefectural University

• Tanaka Hideaki

  Department Of Applied Chemistry & Biochemistry Faculty Of Engineering Kumamoto University

• Iwasaki Yukimoto

  Faculty Of Biotechnology Fukui Prefectural University

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