Purification and Characterization of a New γ-Glutamylmethylamide-dissimilating Enzyme System from Methylophaga sp. AA-30

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概要

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• A γ-glutamylmethylamide (γ-GMA)-dissimilating enzyme system, H protein and L protein, was purified to homogeneity from a cell-free extract of Methylophaga sp. AA-30. H protein contained flavin and had a molecular mass of 360kDa. It consisted of two dissimilar subunits with molecular masses of 55 and 29kDa. L protein had a molecular mass of 38kDa and contained 1 mol of thiol group per mol of protein. Since L protein in the native form was very labile, the protein was treated with 5, 5'-dithiobis(2-nitrobenzoate) to produce a stable but inactive derivative of the protein, which was purified then reactivated with dithiothreitol. The γ-GMA-dissimilating enzyme system catalyzed the formation of glutamate, formaldehyde, 2-ketoglutarate, and ammonia from γ-GMA, 2-ketoglutarate, and ammonia via the two-step reaction shown below.

• 社団法人日本農芸化学会の論文
• 1995-04-23

著者

• HANAI Kazunari

  Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• Hanai Kazunari

  Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology

• Kajiwara Taishin

  Department Of Food Science And Technology Faculty Of Agriculture Kyushu University

• ASAHI Tadashi

  Department of Bioscience, Fukui Prefectural University

• Asahi Tadashi

  Department Of Bioscience Fukui Prefectural University

• Kimura Toshio

  Faculty of Bioresources, Mie University

• Sugahara Isao

  Faculty of Bioresources, Mie University

• Hanai Katsuyuki

  Faculty of Bioresources, Mie University

• Asahi Tadashi

  Faculty of Biotechnology, Fukui Prefectural University

• Sugahara I

  Mie Univ. Mie Jpn

• Sugahara Isao

  Faculty Of Bioresoruces Mie University

• Kimura T

  Department Of Chemistry Shiga University Of Medical Science

• Kimura Tetsuya

  Mie Univ. Mie Jpn

• Hanai Katsuyuki

  Faculty Of Bioresources Mie University

• Kimura Toshio

  Faculty Of Bioresources Mie University

• Asahi Tadashi

  Faculty Of Agriculture Nagoya University

• Kimura Toshio

  Faculty Of Bioresoruces Mie University

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