Primary Metabolic Pathway of Methylamine in Methylophaga sp. AA-30(Microbiology & Fermentation Industry)

概要

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γ-Glutamylmethylamide (γ-GMA) synthetase was detected in crude extracts of Methylophaga sp. AA-30, but neither methylamine dehydrogenase nor N-methylglutamate dehydrogenase was observed. A large amount of γ-GMA was accumulated in the cells when the growth on methanol methylamine was inhibited with iodoacetate, but the accumulation was not observed in the cells grown on methanol-(NH_4)_2SO_4. It is thought that γ-GMA is a metabolic intermediate of the methylamine-dissimilating pathway in the bacterium. In addition, γ-GMA-dissimilating enzymes were found in methylamine-grown cells. The enzymes, which consisted of H protein and L protein, required α-ketoglutaric acid, Mg^<2+> or Mn^<2+>, and ammonia as a cofactor. Although the enzyme catalyzed the formation of glutamate from γ-GMA, it did not catalyze the formation of N-methylglutamate. Consequently, in this bacterium, methylamine seems to be metabolized through a different pathway from the N-methylglutamate pathway.
社団法人日本農芸化学会の論文
1990-11-23