Continuous Production of Maltose Using a Dual Immobilized Enzyme System(Biological Chemistry)

概要

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A dual immobilized enzyme system composed of immobilized β-amylase [EC 3.2.1.2] and pullulanase [EC 3.2.1.41] was studied for the continuous production of maltose. Porous chitosan beads were selected from among many commercial carriers as the best carrier to immobilize β-amylase. The properties of the immobilized enzyme were examined and compared with those of the native enzyme. The optimum pH of the immobilized β-amylase shifted slightly to the alkaline side and the pH stability was improved. The optimum temperature of the immobilized enzyme increased by about 5℃ and the thermostability of the enzyme did not change compared to the native enzyme. Using a dual immobilized enzyme system composed of immobilized β-amylase and pullulanase, the effects of operating conditions on the maltose production reaction were examined. To obtain a maltose content of higher than 75% (w/w), it was necessary to operate at a space velocity of below 0.6hr^<-1> with a substrate concentration of below 25% (w/w) under the optimum reaction conditions. Also, the dual enzyme system proved to be effective in increasing the maltose content in the product.
社団法人日本農芸化学会の論文
1989-12-23