Study of novel d1 alleles, defective mutants of the α subunit of heterotrimeric G-protein in rice

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概要

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• It has been shown that the disruption of the α-subunit gene of heterotorimeric G-proteins (Gα) results in dwarf traits, the erection of leaves and the setting of small seeds in rice. These mutants are called d1. We have studied the expression profiles of the transcripts and translation products of rice Gα in ten alleles of d1 including five additional alleles newly identified. By RT-PCR, the transcripts of the Gα gene were detected in the all d1 alleles. By western blot, the Gα proteins were not detected in the plasma membrane fractions of the d1 alleles with the exception of d1-4. In d1-4, one amino acid change in the GTP-binding box A of the Gα protein was occurred and even in this case the Gα protein was only just detectable in the plasma membrane fraction. Given that the Gα protein did not accumulate in the plasma membrane fraction in d1-8 which has a deletion of just a single amino acid in the Gα protein, it is likely that a proper conformation of the Gα is necessary for accumulation of Gα protein in the plasma membrane. Nine alleles of d1 showed a severer phenotype whilst d1-4 exhibited a mild phenotype with respect to seed size and elongation pattern of internodes. As brassinosteroid signaling was known to be partially impaired in d1s, the sensitivity to 24-epibrassinolide (24-epiBL) was compared among d1 alleles in a T65 genetic background. Only d1-4 showed responses similar to wild type rice. The results show that the d1-4 mutant is a mild allele in terms of the phenotype and mild hyposensitivity to the exogenously applied 24-epiBL.

• 日本遺伝学会の論文
• 2009-02-25

著者

• KITANO Hidemi

  Bioscience and Biotechnology Center, Nagoya University

• Kitano H

  Nagoya Univ. Nagoya Jpn

• Kitano Hidemi

  Grad. Sch. Bioagr. Sci. Nagoya Univ.

• Takahashi Sachiko

  Department Of Bioscience Fukui Prefectural University

• OKI Katsuyuki

  Department of Bioscience, Fukui Prefectural University

• INABA Noriko

  Department of Bioscience, Fukui Prefectural University

• FUJISAWA Yukiko

  Department of Bioscience, Fukui Prefectural University

• KATO Hisaharu

  Department of Bioscience, Fukui Prefectural University

• IWASAKI Yukimoto

  Department of Bioscience, Fukui Prefectural University

• Fujisawa Y

  Department Of Bioscience Fukui Prefectural University

• Fujisawa Yukiko

  Department Of Bioscience Fukui Prefectural University

• Kato Hisaharu

  Department Of Bioscience Fukui Prefectural University

• Inaba Noriko

  Department Of Bioscience Fukui Prefectural University

• Iwasaki Yukimoto

  Department Of Bioscience Fukui Prefectural University

• Iwasaki Yukimoto

  Department Of Bioscience Faculty Of Biotechnology Fukui Prefectural University

• Oki Katsuyuki

  Department Of Bioscience Fukui Prefectural University

• Kitano Hidemi

  Bioscience And Biotechnology Center Nagoya Univ.

• Kitano Hidemi

  Nagoya University School Of Agricultural Science

• Iwasaki Y

  Department Of Bioscience Fukui Prefectural University

• Kitano Hidemi

  Department Of Bioscience Fukui Prefectural University

• Kato Hisaharu

  Department Of Biological Sciences Tokyo Metropolitan University

• Iwasaki Yukimoto

  Faculty Of Biotechnology Fukui Prefectural University

• Kato Hisaharu

  Bioscience and Biotechnology Center, Nagoya University

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