Expression of Elongation Factor 1β'in Escherichia coli and Its Interaction with Elongation Factor 1α from Silk Gland
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概要
- 論文の詳細を見る
Silk gland elongation factor 1 (EF-1) consists of four subunits : α, β, β', and γ. EF-1ββ'γ catalyzes the exchange of GDP for GTP on EF-1α and stimulates the binding of EF-1α-dependent aminoacyl-tRNA to ribosomes. The carboxy-terminal regions of the EF-1β subunits from various species are highly conserved. We examined the region of EF-1β' that binds to EF-1α by in vitro binding assays, and examined the GDP/GTP exchange activity using deletion mutants of a GST-EF1β' fusion protein. We thereby suggested a pivotal amino acid region, residues 189-222,of EF-1β' for binding to EF-1α.
- 社団法人日本農芸化学会の論文
- 1999-04-23
著者
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Yamashita Tetsuro
Department Of Chemical Engineering Faculty Of Engineering Iwate University
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Tamura Hiroto
Dep. Of Environmental Bioscience Meijo Univ.
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Yamashita Tetsuro
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Kobayashi K
Inst. Physical And Chemical Res.(riken) Saitama Jpn
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Taira Hideharu
Department of Bioscience and Technology, Faculty of Agriculture, Iwate University
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Kobayashi K
Department Of Life Science (sports Sciences) The University Of Tokyo
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Taira H
Dep. Of Agro-bioscience Fac. Of Agriculture Iwate Univ.
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Taira Hideharu
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Ejiri S
Cryobiosystem Research Center Faculty Of Agriculture Iwate University
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KIDOU Shin-ichiro
Graduate School of Natural Sciences, Nagoya City University
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KAMIIE Katsuyoshi
Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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KOBAYASHI Kohmei
Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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KIDOU Shinichiro
Cryobiosystem Research Center, Faculty of Agriculture, Iwate University
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EJIRI Shinichiro
Cryobiosystem Research Center, Faculty of Agriculture, Iwate University
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Yamashita T
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Ejiri Shinichiro
Cryobiosystem Res. Cent. Fac. Agr. Iwate Univ.
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Kidou Shinichiro
Graduate School Of Natural Sciences Nagoya City University
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Kidou Shinichiro
Cryobiosystem Res. Cent. Fac. Agr. Iwate Univ.
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