Construction and Characterization of a Fluorescent Sendai Virus Carrying the Gene for Envelope Fusion Protein Fused with Enhanced Green Fluorescent Protein
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概要
- 論文の詳細を見る
Sendai virus (SeV) is an enveloped virus with a non-segmented negative-strand RNA genome. SeV envelope fusion (F) glycoproteins play crucial roles in the viral life cycle in processes such as viral binding, assembly, and budding. In this study, we developed a viable recombinant SeV designated F-EGFP SeV/ΔF, in which the F protein was replaced by an F protein fused to EGFP at the carboxyl terminus. Living infected cells of the recombinant virus were directly visualized by green fluorescence. The addition of EGFP to the F protein maintained the activities of the F protein in terms of intracellular transport to the plasma membrane via the ER and the Golgi apparatus and fusion activity in the infected cells. These results suggest that this fluorescent SeV is a useful tool for studying the viral binding, assembly, and budding mechanisms of F proteins and the SeV life cycle in living infected cells.
- 2010-11-23
著者
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鈴木 幸一
岩手大・農
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Tamura Hiroto
Dep. Of Environmental Bioscience Meijo Univ.
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HASEGAWA Mamoru
DNAVEC Corporation
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Yamashita Tetsuro
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Segawa Hiroaki
Department Of Physiological Chemistry The Tokyo Metropolitan Institute Of Medical Science
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Taira H
Dep. Of Agro-bioscience Fac. Of Agriculture Iwate Univ.
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Taira Hideharu
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Miyazaki Masao
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Zhu Yafeng
Dnavec Corporation
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Segawa H
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Takizawa Kaoru
Dnavec Corporation
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Yamashita T
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Segawa Hiroaki
Department of Agro-Bioscience, Faculty of Agriculture, Iwate University
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