Expression of the Sendai Virus Fusion Protein and the Hemagglutinin-neuraminidase Protein Using a Baculovirus Vector

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概要

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• The fusion (F) and hemagglutinin-neuraminidase (HN) glycoproteins of Sendai virus were expressed using a baculovirus expression vector to examine the structures and functions of these proteins. Both proteins were detected in Spodoptera frugiperda (Sf) cells infected with the recombinant viruses on immunoprecipitation and indirect immunofluorescence staining. The molecular weights of the expressed F and NH proteins were 66 kDa and 70-72 kDa, respectively, suggesting that both proteins were glycosylated, and the F protein was not cleaved into F_1 and F_2 even when the F and HN proteins were coexpressed.

• 社団法人日本農芸化学会の論文
• 1993-04-23

著者

• Iwasaki K

  Kagawa Prefectural Government Kagawa Jpn

• Matsuura Yoshiharu

  Department Of Hygiene And Microbiology Faculty Of Veterinary Medicine Hokkaido University

• IWASAKI Kentaro

  Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoke

• Taira Hideharu

  Department Of Bioscience And Technology Iwate University

• Taira Hideharu

  Department Of Agro-bioscience Faculty Of Agriculture Iwate University

• Matsuura Yoshiharu

  Food Research & Development Laboratories Ajinomoto Co. Inc.

• Sato Toshitsugu

  Department Of Bioscience And Technology Iwate University

• Katsumata Teizo

  Department of Bioscience and Technology, Iwate University

• Katsumata Teizo

  Department Of Bioscience And Technology Iwate University

• Iwasaki Kentaro

  Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science

• KATSUMATA Teizo

  Department of Agricultural Chemistry, Faculty of Agriculture, Iwate University

• Iwasaki Kentaro

  Department of Image Science and Technology, Faculty of Engineering, Chiba University

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