Structural Characterization of N-Glycans of Cauxin by MALDI-TOF Mass Spectrometry and Nano LC-ESI-Mass Spectrometry

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概要

• 論文の詳細を見る

• Cauxin is a carboxylesterase-like glycoprotein excreted as a major component of cat urine. Cauxin contains four putative N-glycosylation sites. We characterized the structure of an N-linked oligosaccharide of cauxin using nano liquid chromatography (LC)-electrospray ionization (ESI) and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS) and MS/MS, and high-performance liquid chromatography (HPLC) with an octadecylsilica (ODS) column. The structure of the N-linked oligosaccharide of cauxin attached to 83Asn was a bisecting complex type, Galβ1-4GlcNAcβ1-2Manα1-3(Galβ1-4GlcNAcβ1-2Manα1-6)(GlcNAcβ1-4)Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc.

• 社団法人 日本農芸化学会の論文
• 2007-03-23

著者

• Yamashita Tetsuro

  Department Of Chemical Engineering Faculty Of Engineering Iwate University

• 鈴木 幸一

  岩手大・農

• Tamura Hiroto

  Dep. Of Environmental Bioscience Meijo Univ.

• SUZUKI Akemi

  Sphingolipid Expression Laboratory, RIKEN Frontier Research System

• Suzuki Akemi

  Sphingolipid Expression Lab. Supra-biomolecular System Res. Group Riken Frontier Res. System

• Yamashita Tetsuro

  Department Of Agro-bioscience Faculty Of Agriculture Iwate University

• Taira Hideharu

  Department of Bioscience and Technology, Faculty of Agriculture, Iwate University

• Taira H

  Dep. Of Agro-bioscience Fac. Of Agriculture Iwate Univ.

• Taira Hideharu

  Department Of Agro-bioscience Faculty Of Agriculture Iwate University

• MIYAZAKI Masao

  Sphingolipid Expression Laboratory, Supra-biomolecular System Group, Frontier Research System, Insti

• Miyazaki Masao

  Department Of Agro-bioscience Faculty Of Agriculture Iwate University

• Ito Emi

  Applied Mass Spectrometry Laboratory Center For Intellectural Property Strategies Riken

• SUZUKI Yusuke

  Sphingolipid Expression Laboratory, Supra-Biomolecular System Research Group, RIKEN Frontier Researc

• SUZUKI Minoru

  Sphingolipid Expression Laboratory, Supra-Biomolecular System Research Group, RIKEN Frontier Researc

• Suzuki Minoru

  Sphingolipid Expression Laboratory Supra-biomolecular System Research Group Riken Frontier Research

• Yamashita T

  Department Of Agro-bioscience Faculty Of Agriculture Iwate University

• Suzuki Yusuke

  Sphingolipid Expression Laboratory Supra-biomolecular System Research Group Riken Frontier Research

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