Disruption of the Membrane-Bound Alcohol Dehydrogenase-Encoding Gene Improved Glycerol Use and Dihydroxyacetone Productivity in Gluconobacter oxydans
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概要
- 論文の詳細を見る
Dihydroxyacetone (DHA) production from glycerol by Gluconobacter oxydans is an industrial form of fermentation, but some problems exist related to microbial DHA production. For example, glycerol inhibits DHA production and affects its biological activity. G. oxydans produces both DHA and glyceric acid (GA) from glycerol simultaneously, and membrane-bound glycerol dehydrogenase and membrane-bound alcohol dehydrogenases are involved in the two reactions, respectively. We discovered that the G. oxydans mutant ΔadhA, in which the membrane-bound alcohol dehydrogenase-encoding gene (adhA) was disrupted, significantly improved its ability to grow in a higher concentration of glycerol and to produce DHA compared to a wild-type strain. ΔadhA grew on 220 g/l of initial glycerol and produced 125 g/l of DHA during a 3-d incubation, whereas the wild-type did not. Resting ΔadhA cells converted 230 g/l of glycerol aqueous solution to 139.7 g/l of DHA during a 3-d incubation. The inhibitory effect of glycerate sodium salt on ΔadhA was investigated. An increase in the glycerate concentration at the beginning of growth resulted in decreases in both growth and DHA production.
- 社団法人 日本農芸化学会の論文
- 2010-07-23
著者
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松下 一信
山口大学農学部生物機能科学科応用微生物学研究室
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Kitamoto Dai
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Fukuoka Tokuma
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Murata K
Dep. Of Basic And Applied Molecular Biotechnology Div. Of Food And Biological Sci. Graduate School O
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Kitamoto Dai
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Sakaki Keiji
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Habe Hiroshi
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Matsushita K
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Takaki Yoshihiro
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Yakushi Toshiharu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Morita Tomotake
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Takashima Masako
Japan Collection Of Microorganisms Riken Bioresource Center
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Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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