Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids
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概要
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A basidiomycetous yeast, Pseudozyma graminicola CBS 10092, was found to accumulate a large amount of glycolipids in the cultured medium when grown on soybean oil as the sole carbon source. Based on thin layer chromatography, the extracellular glycolipids gave spots corresponding to those of mannosylerythritol lipids (MELs), which are highly functional and promising biosurfactants. From the structural characterization by 1H and 13C NMR, the main product was identified as 4-O-[(4′-mono-O-acetyl-2′, 3′-di-O-alka(e)noyl)-β-D-mannopyranosyl]-D-erythritol, which is a highly hydrophilic derivative of MELs known as MEL-C. According to high-performance liquid chromatography analysis, the main product, MEL-C, comprised approximately 85% of all the MELs, and the total amount reached approximately 10 g/L for 7 days. The fatty acids of the present MEL-C consisted of mainly C6, C8 and C14 acids, considerably different from those of MEL-C produced by other Pseudozyma strains such as P. antarctica and P. shanxiensis. The observed critical micelle concentration (CMC) and the surface-tension at CMC of the MEL-C were 4.0 × 10-6 M and 24.2 mN/m, respectively, while those of MEL-A, the most intensively studied MEL, were 2.7 × 10-6 M and 28.4 mN/m, respectively. This implied that the MEL-C has higher hydrophilicity than conventional MELs hitherto reported. In addition, on a water-penetration scan, the MEL-C efficiently formed the lamella phase (Lα) at a wide range of concentrations, indicating its excellent self-assembling properties. From these results, the newly identified MELs produced by P. graminicola are likely to have great potential for use in oil-in-water type emulsifiers and/or washing detergents, and would thus facilitate a broad range of applications for the promising yeast biosurfactants.
- 日本油化学会の論文
著者
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KITAMOTO Dai
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria
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Imura Tomohiro
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria
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Fukuoka Tokuma
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria
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Konishi Masaaki
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria
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Morita Tomotake
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria
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Konishi Masaaki
Res. Inst. For Innovation In Sustainable Chemistry National Inst. Of Advanced Industrial Sci. And Te
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Kitagawa Masaru
Toyobo Co., Ltd. Corporate Research Center
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Yamamoto Shuhei
Toyobo Co., Ltd. Corporate Research Center
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Sogabe Atsushi
Toyobo Co., Ltd. Corporate Research Center
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Fukuoka Tokuma
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Imura Tomohiro
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Kitamoto Dai
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Morita Tomotake
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Takashima Masako
Japan Collection Of Microorganisms Riken Bioresource Center
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Sogabe Atsushi
Toyobo Research Center
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Kitagawa Masaru
Toyobo Research Center
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Yamamoto Shuhei
Toyobo Research Center
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IMURA Tomohiro
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
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MORITA Tomotake
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
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