Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung(Biopharmacy)

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概要

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• As reported previously, cationic liposomes formulated with dioleoylphosphatidylethanolamine (DOPE) and N,N-methyl hydroxyethyl aminopropane carbamoyl cholesterol (MHAPC-liposomes) achieved efficient gene transfection in the mouse lung following intratracheal injection. We have studied here the role of surfactants, mannosylerythritol lipid-A (MEL-A) and polysorbate 80 (Tween 80), in affecting gene transfection of MHAPC-lipoplexes (complex with pCMV-luc DNA) in A549 cells and in the mouse lung. MEL-A increased gene transfection of MHAPC-lipoplexes significantly in vitro and slightly in the mouse lung, while Tween 80 decreased it both in vitro and in vivo. As assessed by confocal laser scanning microscopy and fluorescence imaging, MEL-A might faciliate gene dissociation from MHAPC-lipoplexes with fluorescein-labeled oligodeoxynucleotide (FITC-ODN) after internalization into the cells and retained the lipoplexes in the mouse lung for prolonged time, while Tween 80 was inefficient to deliver foreign gene into target cells and in the lung. These results demonstrated that MEL-A is advantageous to Tween 80 in the modification of cationic liposomes as gene delivery vectors in the lung.

• 2009-02-01

著者

• DING Wuxiao

  Institute of Medicinal Chemistry, Hoshi University

• IZUMISAWA Tomohiro

  Institute of Medicinal Chemistry, Hoshi University

• HATTORI Yoshiyuki

  Institute of Medicinal Chemistry, Hoshi University

• QI Xianrong

  Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University

• KITAMOTO Dai

  Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria

• MAITANI Yoshie

  Institute of Medicinal Chemistry, Hoshi University

• Kitamoto Dai

  Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An

• Maitani Y

  Institute Of Medicinal Chemistry Hoshi University

• Maitani Yoshie

  星薬科大学医薬品化学研究所

• Maitani Yoshie

  Institute Of Medicinal Chemistry Hoshi University

• Qi Xianrong

  Department Of Pharmaceutics School Of Pharmaceutical Sciences Peking University

• Kitamoto Dai

  Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial

• Hattori Yoshiyuki

  星薬科大学医薬品化学研究所

• Hattori Yoshiyuki

  Institute Of Medicinal Chemistry Hoshi University

• Maitani Yoshie

  Faculty Of Pharmaceutical Sciences Hoshi University

• Ding Wuxiao

  Institute Of Medicinal Chemistry Hoshi University

• Izumisawa Tomohiro

  Institute Of Medicinal Chemistry Hoshi University

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