Electron Spin Resonance Studies of Dipalmitoylphosphatidylcholine Liposomes Containing Soybean-Derived Sterylglucoside

概要

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The effects of soybean-derived sterylglucoside (SG) on the fluidity of liposomal membrane composed of dipalmitoylphosphatidylcholine (DPPC) were investigated compared with those of soybean-derived sterol (SS) and cholesterol (Ch) using an electron spin resonance spectrometer. Three kinds of liposomes were prepared in the molar ratio of DPPC/X=7/4,where X is SS, Ch or SG. The fluidity close to the polar head groups increased with an increase of temperature in the DPPC membrane containing SS, Ch and SG in the range 35 to 45℃. Those near the hydrophobic end changed with an increase in temperature in liposomes containing SS, Ch and SG, which had a fluidizing effect on the DPPC membrane below the transition temperature (Tm, 41.9℃) and a condensing effect over the Tm. The fluidizing effects of these compounds around 37℃ near the polar head group and the hydrophobic end increased in the following order : Ch<SG≤SS and SS<Ch<SG, respectively. SG increased the fluidity of liposomal membrane dramatically above the Tm (35.4℃). These results suggest that the high fluidity close to the hydrophobic end of the liposomal membranes around 37℃, the decrease of Tm, and the sigmoidal nature of fluidity vs. temperature are important factors in the effectiveness of liposomes containing SG as a carrier of drugs.
公益社団法人日本薬学会の論文
2000-05-01