High Entrapment of Insulin and Bovine Serum Albumin into Neutral and Positively-Charged Liposomes by the Remote Loading Method
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概要
- 論文の詳細を見る
Remote loading of insulin and bovine serum albumin (BSA) into neutral or positively-charged liposomes by incubation under a transmembrane pH gradient or non-pH gradient was investigated. Trapping efficiencies in several incubation conditions were compared with those of the conventional reverse-phase evaporation vesicle method (c-REV method). For neutral liposomes, insulin could not be effectively loaded into the liposomes by incubation, regardless of the incubation conditions.The trapping efficiency of insulin into positively-charged liposomes was higher than that of neutral liposomes, especially by the pH-gradient method. Insulin could be loaded into positively-charged liposomes about twofold more efficiently than by the pH-gradient method, compared with the c-REV method. Insulin distributed more on the surface of liposomes by the pH-gradient method than by the c-REV method. BSA showed significantly higher affinity to positively-charged liposomes than to neutral ones by various methods. However, the transmembrane pH-gradient method did not increase BSA loading into liposomes, compared with the c-REV and the non-pH-gradient methods. Our results suggest that the pH-gradient method, combining electrostatic interactions, may be useful for preparation of liposomal insulin and that the high hydrophobicity of BSA may not increase the remote loading of BSA into liposomes by the pH-gradiet method.
- 公益社団法人日本薬学会の論文
- 2000-03-01
著者
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HWANG Sung
Department of Electronics Engineering, Korea University
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Takayama Kozo
Department of Pharmaceutics, Hoshi University
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Maitani Y
Institute Of Medicinal Chemistry Hoshi University
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Maitani Yoshie
Department Of Pharmaceutics Hoshi University
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Nagai T
Department Of Pharmaceutics Hoshi University
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Hwang S
Hoshi Univ. Tokyo Jpn
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Takayama Kozo
Department Of Pharmaceutics Hoshi University
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Takayama Kozo
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Hoshi University
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NAGAI TSUNEJI
Department of Pharmaceutics, Hoshi University
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Hwang Sung
Department Of Electronics And Computer Engineering Korea University
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Takayama Kozo
Dep. Of Pharmaceutics Hoshi Univ.
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Maitani Yoshie
Faculty Of Pharmaceutical Sciences Hoshi University
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Nagai Tsuneji
Department Of Pharmaceutics Hoshi University
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Takayama K
Hoshi Univ. Tokyo Japan.
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Hwang Sung
Department of Electronics & Computer Engineering, Korea University, Anam, Sungbuk, Seoul 136-701, Korea
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Takayama Kozo
Department of Drug Delivery Research, Hoshi University
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Maitani Yoshie
Department of Drug Delivery Research, Hoshi University
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Hwang Sung
Department of Electronic and Computer Engineering, Korea University, Anam, Sungbuk, Seoul 136-075, Korea
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Nagai Tsuneji
Department of Drug Delivery Research, Hoshi University
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Hwang Sung
Department of Chemistry, Sahmyook University
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