Association of Liposomes Containing a Soybean-Derived Sterlyglucoside Mixture with Rat Primary Cultured Hepatocytes
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概要
- 論文の詳細を見る
Dipalmitoylphosphatidylcholine (DPPC) and cholesterol (Ch) liposomes containing a soybean-derived sterylglucoside mixture (SG) (SG-liposomes) are effective for targeting hepatocytes in mice. We investigated uptake of SG-liposomes to hepatocytes compared with that via the galactose receptor. The association of SG-liposomes entrapping calcein was examined at 4℃or 37℃, changing incubation time and the SG concentration of the liposomes, and using the inhibitor on the galactose receptor in rat primary cultured hepatocytes. The amount of liposomes recovered with hepatocytes was determined by measuring the concentration of calcein and imaged with confocal laser scanning microscopy in the cultured cells. The association of SG-liposomes at 4℃ was significantly decreased compared with that at 37℃ as that of liposomes containing lactosylceramide (LC) (LC-liposomes) that were already known to be taken up by hepatocytes via the asialogycoprotein receptor. While the association of SG-liposomes at both 4℃ and 37℃ increased with the increase of incubation time, the association of SG-liposomes at 37℃ was almost saturated after 1 h. The association of SG-liposomes pretreated with concanavalin A was significantly decreased (to about 40% of that without pretreatment at 37℃) and was at the same level as the association of SG-liposomes and non-SG-liposomes at 4℃. The association of the SG-liposomes by hepatocytes incubated for 1 h at 37℃ was almost saturated at about 2.0 nmol SG/mg protein. The association of the SG-liposomes with hepatocytes was not inhibited in the presence of LC-liposomes. The affinity of the galactose residue for hepatocyts appeared to be similar to that of the glucose residue in the liposomes, because the amount of sugar residue and the association of SG-liposomes and LC-liposomes were slmost the same values. These results suggest that SG-liposomes may be associated with hepatocytes not by a galactose receptor-mediated endocytosis.
- 公益社団法人日本薬学会の論文
- 1998-08-15
著者
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Takahashi Noriko
Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences Core Research Fo
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Takayama Kozo
Department of Pharmaceutics, Hoshi University
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Shimizu Kazuyuki
Department Of Biochemical Engineering & Science Kyushu Institute Of Technology
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Shimizu K
Department Of Pharmaceutics Hoshi University
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Maitani Y
Institute Of Medicinal Chemistry Hoshi University
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Maitani Yoshie
Department Of Pharmaceutics Hoshi University
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Nagai T
Department Of Pharmaceutics Hoshi University
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Takayama K
Hoshi Univ. Tokyo Jpn
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Takayama Kozo
Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Hoshi University
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NAGAI TSUNEJI
Department of Pharmaceutics, Hoshi University
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Takayama Kozo
Dep. Of Pharmaceutics Hoshi Univ.
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SHIMIZU Kazuyuki
Faculty of Pharmaceutical Sciences, Hoshi University
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MAITANI Yosie
Department of Pharmaceutics Hoshi University
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Maitani Yoshie
Faculty Of Pharmaceutical Sciences Hoshi University
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Nagai Tsuneji
Department Of Pharmaceutics Hoshi University
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Shimizu Kazuyuki
Faculty Of Pharmaceutical Sciences Hoshi University
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Shimizu Kazuyuki
Department Of Biochem.engineering And Science Kyushu Institute Of Technology
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Takahashi Noriko
Department Of Health Chemistry Hoshi University
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Takayama Kozo
Department of Drug Delivery Research, Hoshi University
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Maitani Yoshie
Department of Drug Delivery Research, Hoshi University
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Shimizu Kazuyuki
Department of Applied Chemistry for Resources, Tokyo University of Agriculture and Technology
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Nagai Tsuneji
Department of Drug Delivery Research, Hoshi University
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