Efficient Production of Gene Products by Repeated Fed-Batch Fermentation Using Multiple Fermentors
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概要
- 論文の詳細を見る
In the coltivation of genetically engineered microorganisms, cell growth decreases after induction, and it becomes eminent when a product such as β-galactosidase is harvested, indicating that conventional repeated fed-batch operation cannot be used to increase productivity. In this study, the use of multiple fermentors to overcome such difficulties was attempted. A mathematical model was developed based on fed-batch experiments using Escherichia coli JM103 harboring the pUR2921 plasmid encoding the β-galactosidase structural gene. Computer simulation demonstrated that improved performance can be attained by repeated fed-batch operation using multiple fermentors. Experiments were then conducted to ascertain the operating conditions.
- 公益社団法人日本生物工学会の論文
- 1994-02-25
著者
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Shimizu Kazuyuki
Department Of Biochemical Engineering & Science Kyushu Institute Of Technology
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Shimizu Kazuyuki
Department Of Biochem.engineering And Science Kyushu Institute Of Technology
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FUJIOKA KIYOSHI
Department of Biochemical Engineering & Science, Kyushu Institute of Technology
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Fujioka Kiyoshi
Department Of Biochemical Engineering & Science Kyushu Institute Of Technology
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Shimizu Kazuyuki
Department of Applied Chemistry for Resources, Tokyo University of Agriculture and Technology
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