Temperature-Induced Expression of phb Genes in Escherichia coli and the Effect of Temperature Patterns on the Production of Poly-3-Hydroxybutyrate
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概要
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The plasmid pNKTM2 was constructed to contain the p_R-p_L promoter and the phbC, A, and B genes which code for PHB synthase, β-ketothiolase, and acetoacetyl-CoA reductase, respectively. This plasmid was transformed into Escherichia coli XL1-Blue. The effect of several thermal induction patterns on the production of poly-3-hydroxybutyric acid (PHB) was investigated based on fermentor experiments. Based on the experimental results with different induction patterns, it was found that the temperature should be controlled at 34℃ during the initial 10 h of cultivation of promote cell growth, and then it should be increased to 40℃ for induction. Then the temperature should be lowered to 37℃ after 5 h to relieve the effect of the heat shock.
- 公益社団法人日本生物工学会の論文
- 2001-01-25
著者
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Shi Huidong
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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Shimizu Kazuyuki
Department Of Biochem.engineering And Science Kyushu Institute Of Technology
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Takasu Mitsuhiro
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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KYUWA KAORU
Department of Biochemical Engineering and Science, Kyushu Institute of Technology
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Kyuwa Kaoru
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
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