Proteome Analysis of a Temperature-Inducible Recombinant Escherichia coli for Poly-β-Hydroxybutyrate Production
スポンサーリンク
概要
- 論文の詳細を見る
A recombinant Escherichia coil strain harboring the λp_R-p_L promoter and heterologous poly-β-hydroxybutyrate(PHB)biosynthesis genes was shown to accumulate PHB when the incubation temperature was changed from 34℃ to temperatures higher than 37℃. In the present research, total gene expression patterns of the recombinant E.coli before and after induction were investigated by two-dimensional gel electrophoresis. Proteins encoded by serS, sucC, trpA, and alaS were found to be expressed before induction of phb genes at a culture temperature of 34℃. On the other hand, proteins encoded by metG, rplI, and carA were found to be expressed after induction achieved by increasing the temperature to 40℃. In the case of plasmid-free cells, all the selected genes have been shown to be expressed except metG, and ibpA and ibpB among the heat-shock proteins. The heat-shock proteins were found to be upregulated upon induction of phb genes, which may be due to the stress caused by the accumulation of PHB granules as well as by the temperature upshift. The changes in the expression of some of the metabolic pathway-related proteins before and after induction were interpreted in relation to the consumption of NADPH and acetyl-CoA for PHB synthesis.
- 社団法人日本生物工学会の論文
- 2001-09-25
著者
-
Shimizu Kazuyuki
Department Of Biochemical Engineering & Science Kyushu Institute Of Technology
-
Kabir Md.
Department Of Chemistry Graduate School Of Science Osaka University
-
Kabir Md.mohiuddin
Department Of Biochemical Engineering And Science Kyushu Institute Of Technology
-
Shimizu Kazuyuki
Department Of Biochem.engineering And Science Kyushu Institute Of Technology
-
Kabir Md.
Department of Biochemical Engineering and Science, Kyushu Institute of Technology
-
Shimizu Kazuyuki
Department of Applied Chemistry for Resources, Tokyo University of Agriculture and Technology
関連論文
- Metabolic Pathway Analysis of Recombinant Saccharomyces cerevisiae with a Galactose-Inducible Promoter Based on a Signal Flow Modeling Approach
- Phase II and pharmacokinetic study of thalidomide in Japanese patients with relapsed/refractory multiple myeloma
- Quantitative Analysis of Intracellular Metabolic Fluxes Using GC-MS and Two-Dimensional NMR Spectroscopy
- Metabolic Control Analysis for Lysine Synthesis Using Corynebacterium glutamicum and Experimental Verification
- Development of a Kinetic Model for L-Lysine Biosynthesis in Corynebacterium glutamicum and Its Application to Metabolic Control Analysis
- Metabolic Flux Analysis for Efficient Pyruvate Fermentation Using Vitamin-Auxotrophic Yeast of Torulopsis glabrata
- Metabolic Flux Analysis for Biosynthesis of Poly(β-Hydroxybutyric Acid) in Alcaligenes eutrophus from Various Carbon Sources
- Efficient Production of Vitamin B_ from Propionic Acid Bacteria under Periodic Variation of Dissolved Oxygen Concentration
- Application of Artificial Neural Network and Fuzzy Control for Fed-Batch Cultivaton of Recombinant Saccharomyces cerevisiae
- Performance Improvement of Lactic Acid Fermentation by Multistage Extractive Fermentation
- 753 Design and Evaluation of Extractive Fermentation with Hollow Fiber Module for Lactic Acid Production
- 752 Overexpression of Plasmid Encoded Protein in S. cerevisiae with GAL 10 Promoter
- Fuzzy Neural Network for the Control of High Cell Density Cultivation or Recombinant Escherichia coli
- 520 Neuro-fuzzy Control of Recombinant E. Coli Focucing on the Metabolic Regulation
- Effect of Controlling Lactate Concentration and Periodic Change in DO Concentration on Fermentation Characteristics of a Mixed Culture of Lactobacillus delbrueckii and Ralstonia eutropha for PHB Production
- Rapid Production of Pediococcus halophilus Salt-Tolerant Cells by a Cultivation Method Employing Gradual Increases in NaCl Concentration Using a Fermentor with a Microfiltration Module
- Posttreatment M-Protein Nadir Level Is a Significant Prognostic Factor Associated with Survival in Multiple Myeloma
- Continuous Lactic Acid Production from Raw Starch in a Fermentation System Using a Reversibly Soluble-autoprecipitating Amylase and Immobilized Cells of Lactobacillus casei(Microbiology & Fermentation Industry)
- Development of Dual On-Line Analyzer and Its Application to Fed-Batch Lactic Acid Fermentation
- Adaptive On-Line Optimizing Control for Lactic Acid Fermentation
- pH-Mediated Control Methods for Continuous Ethanol Fermentation Using Zymomonas mobilis
- Design and Construction of Coordination Polymers Based on the Topological Property of the Multidentate Ligand
- Hodgkin's disease following extranodal marginal zone B-cell lymphoma in remission
- Targeting of Soybean-Derived Sterylglucoside Liposomes to Liver Tumors in Rat and Mouse Models
- Formulation of Liposomes with a Soybean-Derived Sterylglucoside Mixture and Cholesterol for Liver Targeting
- Neuro-Fuzzy Control of Bioreactor Systems with Pattern Recognition
- On-Line Control of Glucose Concentration Using An Automatic Glucose Analyzer
- Association of Liposomes Containing a Soybean-Derived Sterlyglucoside Mixture with Rat Primary Cultured Hepatocytes
- Knowledge-Based Design and Operation of Bioprocess Systems
- A Novel Ruthenium-Quinonoid Complex. Structural, Spectroscopic, and Electrochemical Characterization of Ruthenium(II) Bis(2,2'-bipyridine) Chloranilate
- Proteome Analysis of a Temperature-Inducible Recombinant Escherichia coli for Poly-β-Hydroxybutyrate Production
- An Integrated Metabolic Pathway Analysis Based on Metabolic Signal Flow Diagram and Cellular Energetics for Saccharomyces cerevisiae
- 571 Computer-mediated addition of fresh medium in continuous culture of Zymomonas mobilis by the monitoring of weight changes
- Hepatic Branched-Chain α-Keto Acid Dehydrogenase Complex in Female Rats : Activation by Exercise and Starvation
- Application of Dual-Energy K-Edge Subtraction Imaging to Assessment of Heat Treatments in Al-Cu Alloys
- Two-wave mixing amplification of femtosecond laser pulses at 800 nm in Rh:BaTiO3 photorefractive crystal
- Modification of Metabolic Pathways of Saccharomyces cerevisiae by the Expression of Lactate Dehydrogenase and Deletion of Pyruvate Decarboxylase Genes for the Lactic Acid Fermentation at Low pH Value
- Effect of Modifying Metabolic Network on Poly-3-Hydroxybutyrate Biosynthesis in Recombinant Escherichia coli
- Temperature-Induced Expression of phb Genes in Escherichia coli and the Effect of Temperature Patterns on the Production of Poly-3-Hydroxybutyrate
- Efficient Production of Gene Products by Repeated Fed-Batch Fermentation Using Multiple Fermentors
- High-Mg garnets from pelitic schists adjacent to the Sebadani eclogitic metagabbro mass, Sambagawa metamorphic belt, central Shikoku, Japan
- Genetic Diversity in Three Forms of Anabas testudineus Bloch
- Solvent extraction of plant pigments from leaf protein concentrate.
- N-Hydroxy amides. IV Synthesis and properties of a trihydroxamic acid anilide as a model for ferrioxamines.
- N-hydroxy amides. I. Synthesis of N-benzyloxy and N-hydroxy peptides via polymerization of N-benzyloxy DL-.ALPHA.-amino acid N-carboxy anhydrides.
- Comparison of control techniques for baker's yeast culture using an automatic glucose analyzer.
- Metamorphic pressure-temperature evolution of garnet-chloritoid schists from the Lake Zone, SW Mongolia
- N-hydroxy amides. III. Active esters of polystyrene-bound 1-hydroxy-2-pyrrolidinone and their use in peptide synthesis.
- On the expansion of the transfer function for the model reduction of stage systems.
- Singular value analysis for the Robust control system design of distillation systems.
- Control of cell and lactate concentration in a hollow-fiber bioreactor for lactic acid fermentation.