Surface Properties of Lipoplexes Modified with Mannosylerythritol Lipid-A and Tween 80 and Their Cellular Association
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概要
- 論文の詳細を見る
The surface properties of cationic liposomes and lipoplexes largely determine the cellular association and gene transfection efficiency. In this study, we measured the surface properties, such as zeta potentials, surface pH and hydration levels of MHAPC- and OH-Chol-lipoplexes and their cellular association, without and with the modification of biosurfactant mannosylerythritol lipid-A (MEL-A) or Tween 80 (MHAPC=N,N-methyl hydroxyethyl aminopropane carbamoyl cholesterol; OH-Chol=cholesteryl-3β-carboxyamindoethylene-N-hydroxyethyl-amine). Compared to OH-Chol-lipoplexes, the higher cellular association of MHAPC-lipoplexes correlated with the significantly higher zeta potentials, lower surface pH levels and "drier" surface, as evaluated by the generalized polarization of laurdan. Both MEL-A and Tween 80 modification of MHAPC-lipoplexes did not significantly change zeta potentials and surface pH levels, while MEL-A modification of OH-Chol-lipoplexes seriously decreased them. MEL-A hydrated the liposomal surface of MHAPC-lipoplexes but dehydrated that of OH-Chol-lipoplexes, while Tween 80 hydrated those of MHAPC- and OH-Chol-lipoplexes. In all, cationic liposomes composed of lipids with secondary and tertiary amine exhibited different surface properties and cellular associations of lipoplexes, and modification with surfactants further enlarged their difference. The strong hydration ability of Tween 80 may relate to the low cellular association of lipoplexes, while the dehydration of MEL-A-modified OH-Chol-lipoplexes seemed to compensate the negative zeta potential for the cellular association of lipoplexes.
- 2009-02-01
著者
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DING Wuxiao
Institute of Medicinal Chemistry, Hoshi University
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HATTORI Yoshiyuki
Institute of Medicinal Chemistry, Hoshi University
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QI Xianrong
Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University
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KITAMOTO Dai
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industria
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MAITANI Yoshie
Institute of Medicinal Chemistry, Hoshi University
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Kitamoto Dai
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Maitani Y
Institute Of Medicinal Chemistry Hoshi University
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Maitani Yoshie
星薬科大学医薬品化学研究所
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Maitani Yoshie
Institute Of Medicinal Chemistry Hoshi University
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Qi Xianrong
Department Of Pharmaceutics School Of Pharmaceutical Sciences Peking University
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Kitamoto Dai
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Hattori Yoshiyuki
星薬科大学医薬品化学研究所
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Hattori Yoshiyuki
Institute Of Medicinal Chemistry Hoshi University
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Maitani Yoshie
Faculty Of Pharmaceutical Sciences Hoshi University
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Ding Wuxiao
Institute Of Medicinal Chemistry Hoshi University
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