DNA/Lipid Complex Incorporated with Fibronectin to Cell Adhesion Enhances Transfection Efficiency in Prostate Cancer Cells and Xenografts(Biopharmacy)
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概要
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Previously we have described the development and applications of lipid-based nanoparticles for gene delivery vector. In an attempt to improve transfection efficiency using the cell adhesion of extracellular matrix (ECM) to DNA/lipid complex (nanoplex), the mRNA expression of integrin α2β1 and CD44 in prostate cancer cells was detected as adhesion molecules for fibronectin (Fn), collagen I (Col) and laminin (Lam) using a commercially available cDNA array (GEArray^<TM>) system. These ECM proteins could enhance DNA transfection activity in cells when coated on the nanoplex. Among the ECM proteins, Fn-coating nanoplexes significantly increased transfection activity 2-fold in prostate cancer PC-3 cells, and exhibited higher DNA transfection activities to PC-3 xenografts, compared with commercially available cationic polymer in vivo jetPEI. These results indicated that Fn-coating nanoplexes could facilitate efficient transfection of prostate tumor cells.
- 公益社団法人日本薬学会の論文
- 2007-03-01
著者
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HATTORI Yoshiyuki
Institute of Medicinal Chemistry, Hoshi University
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MAITANI Yoshie
Institute of Medicinal Chemistry, Hoshi University
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Maitani Yoshie
Institute Of Medicinal Chemistry Hoshi University
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Hattori Yoshiyuki
星薬科大学医薬品化学研究所
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Hattori Yoshiyuki
Institute Of Medicinal Chemistry Hoshi University
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Maitani Yoshie
Institute Of Medicinal Chemistry Hoshi Universty
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