Membrane-Bound Alcohol Dehydrogenase Is Essential for Glyceric Acid Production in Acetobacter tropicalis
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概要
- 論文の詳細を見る
Acetobacter tropicalis NBRC16470 can produce highly enantiomerically pure D-glyceric acid (D-GA; >99 % enantiomeric excess) from glycerol. To investigate whether membrane-bound alcohol dehydrogenase (mADH) is involved in GA production in A. tropicalis, we amplified part of the gene encoding mADH subunit I (adhA) using polymerase chain reaction and constructed an adhA-disrupted mutant of A. tropicalis (ΔadhA). Because ΔadhA did not produce GA, we confirmed that mADH is essential for the conversion of glycerol to GA. We also cloned and sequenced the entire region corresponding to adhA and adhB, which encodes mADH subunit II. The sequences showed high identities (84–86 %) with the equivalent mADH subunits from other Acetobacter spp.
- 日本油化学会の論文
著者
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Fukuoka Tokuma
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Science An
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Kitamoto Dai
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Matsushita Kazunobu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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Sakaki Keiji
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Habe Hiroshi
Research Institute For Innovation In Sustainable Chemistry National Institute Of Advanced Industrial
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Sato Shun
Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industri
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Yakushi Toshiharu
Department Of Biological Chemistry Faculty Of Agriculture Yamaguchi University
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KITAMOTO Dai
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
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Sakaki Keiji
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
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Habe Hiroshi
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
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Matsushita Kazunobu
Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University
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Sato Shun
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
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